Interleukin-18 synthesis and secretion by dendritic cells are modulated by interaction with antigen-specific T cells
Interleukin-18 synthesis and secretion by dendritic cells are modulated by interaction with antigen-specific T cells
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DOI：
10.1002/jlb.66.2.237
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发表时间：
1999-08-01
影响因子：
5.5
通讯作者：
Rubartelli, A
中科院分区：
文献类型：
--
作者：
Gardella, S;Andrei, C;Rubartelli, A
我们发现白细胞介素-18是由树突状细胞组成的；成熟刺激对树突状细胞的合成和分泌影响较小，自体抗破伤风环形T淋巴细胞攻击树突状细胞导致分泌开关，诱导生物活性白介素-18的分泌并减少其细胞内含量，同样，当树突状细胞受到同种异体T细胞攻击时，细胞内白介素-18含量急剧下降。而与自体活化T细胞共培养后，未观察到任何影响。CD40抗体触发CD40后，树突状细胞上清液中白细胞介素-18的含量增加，这表明CD40与树突状细胞的结合可以介导白细胞介素-18的分泌。然而，与抗原特异性T细胞不同，CD40的结合不会导致细胞内白细胞介素-18含量减少，这表明这种减少可能是由参与抗原识别的结构介导的。
We show that interleukin-18 is constitutively produced by dendritic cells; synthesis and secretion are poorly affected by maturative stimuli, Challenge of dendritic cells with autologous antitetanus toroid T lymphocytes results in a secretory switch, with induction of secretion of biologically active interleukin-18 and decrease of its intracellular content, Similarly, when dendritic cells are challenged with allospecific T cells a dramatic decrease of intracellular interleukin-18 content occurs, whereas no effects are observed after co-culture with autologous activated T cells. The induction of secretion can be mediated by engagement of CD40 on dendritic cells, as indicated by the increased amount of interleukin-18 in dendritic cell supernatants after CD40 triggering by anti-CD40 antibodies, However, CD40 engagement, unlike from antigen-specific T cells, does not result in reduced intracellular interleukin-18 content, suggesting that this decrease may be mediated by structure(s) involved in antigen recognition.