Mef2d acts upstream of muscle identity genes and couples lateral myogenesis to dermomyotome formation in Xenopus laevis.
Mef2d acts upstream of muscle identity genes and couples lateral myogenesis to dermomyotome formation in Xenopus laevis.
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DOI：
10.1371/journal.pone.0052359
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Chanoine C
中科院分区：
文献类型：
--
作者：
Della Gaspera B;Armand AS;Lecolle S;Charbonnier F;Chanoine C
非洲爪蟾肌瘤是由第一次直接起源于体前中胚层的内侧和外侧肌形成，然后由皮切面发出的第二次肌形成波形成的。在这里，通过一系列的功能增益和损失实验，我们发现Mef2d， MADS-box转录因子Mef2家族的成员，作为外侧肌发生的上游调节剂，以及在神经发育开始时作为皮组织形成的诱导剂。在外侧体前细胞中，我们发现Mef2d激活Myod的表达，这是外侧肌形成所必需的。在大多数体前中胚层的外侧细胞中，我们发现Mef2d和转录因子Twist家族成员Paraxis （Tcf15）共定位并直接激活Meox2的表达，而Meox2在皮组织形成过程中作用于Pax3的上游表达。细胞追踪实验证实，体裂前中胚层中最外侧表达Meox2的细胞与皮细胞组祖细胞相对应，因为它们产生了体裂中胚层中最多的背侧细胞。因此，爪蟾Mef2d将外侧肌发生与表皮组织形成结合在一起，然后再进行体分割。这些结果与我们之前的工作一起揭示了非洲爪蟾皮肌组和肌腱形成之间惊人的相似性：两者都与肌源性细胞相关，并且都涉及基因转激活途径，其中Mef2家族的一个成员Mef2d或Mef2c分别与Twist家族的bHLH蛋白Paraxis或Scx （sclcleraxis）合作。我们认为，非洲爪蟾的这些共同特征反映了一种脊椎动物祖先机制的存在，该机制将肌源性细胞的发育与somite区隔过程中相关组织的形成相结合。
Xenopus myotome is formed by a first medial and lateral myogenesis directly arising from the presomitic mesoderm followed by a second myogenic wave emanating from the dermomyotome. Here, by a series of gain and loss of function experiments, we showed that Mef2d, a member of the Mef2 family of MADS-box transcription factors, appeared as an upstream regulator of lateral myogenesis, and as an inducer of dermomyotome formation at the beginning of neurulation. In the lateral presomitic cells, we showed that Mef2d transactivates Myod expression which is necessary for lateral myogenesis. In the most lateral cells of the presomitic mesoderm, we showed that Mef2d and Paraxis (Tcf15), a member of the Twist family of transcription factors, were co-localized and activate directly the expression of Meox2, which acts upstream of Pax3 expression during dermomyotome formation. Cell tracing experiments confirm that the most lateral Meox2 expressing cells of the presomitic mesoderm correspond to the dermomyotome progenitors since they give rise to the most dorsal cells of the somitic mesoderm. Thus, Xenopus Mef2d couples lateral myogenesis to dermomyotome formation before somite segmentation. These results together with our previous works reveal striking similarities between dermomyotome and tendon formation in Xenopus: both develop in association with myogenic cells and both involve a gene transactivation pathway where one member of the Mef2 family, Mef2d or Mef2c, cooperates with a bHLH protein of the Twist family, Paraxis or Scx (Scleraxis) respectively. We propose that these shared characteristics in Xenopus laevis reflect the existence of a vertebrate ancestral mechanism which has coupled the development of the myogenic cells to the formation of associated tissues during somite compartmentalization.
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DOI：
10.1242/dev.037689
发表时间：
2010-11
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Dorey K;Amaya E
通讯作者：
Amaya E
影响因子：
2.7
作者：
Geiser, M;Cèbe, R;Schmitz, R
通讯作者：
Schmitz, R
影响因子：
4.6
作者：
Grimaldi, A;Tettamanti, G;Hughes, SM
通讯作者：
Hughes, SM
影响因子：
64.8
作者：
Freitas, Renata;Zhang, GuangJun;Cohn, Martin J.
通讯作者：
Cohn, Martin J.
DOI：
10.1387/ijdb.082747rb
发表时间：
2009
期刊：
The International journal of developmental biology
影响因子：
--
作者：
Barnes RM;Firulli AB
通讯作者：
Firulli AB