In vivo generation of cartilage from periosteum
In vivo generation of cartilage from periosteum
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DOI：
10.1089/ten.2005.11.369
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发表时间：
2005-03-01
影响因子：
--
通讯作者：
Kuijer, R
中科院分区：
文献类型：
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作者：
Emans, PJ;Surtel, DAM;Kuijer, R
骨膜具有成软骨和成骨潜能，在骨折愈合中起着重要作用。本研究的目的是评估骨膜损伤后形成的反应性组织。破坏骨膜可能是产生异位软骨或骨的一种方式，这可能对关节软骨和骨缺损的修复有用。从新西兰大白兔胫骨内侧近端两侧剥离骨膜。术后10、20和40天采集反应性骨膜组织，分析I型、II型和X型胶原、聚集蛋白、骨桥蛋白和骨连接蛋白（通过逆转录聚合酶链反应）和I型和II型胶原（通过免疫组织化学）的表达。93%的病例存在反应性组织。组织学上，在随访第10天和第20天，该组织由透明软骨组成。术后10、20 d可见ⅱ型胶原和聚集蛋白的表达。10 d表达量最高。X型胶原蛋白的表达增加到20天。骨桥蛋白和骨连接蛋白mRNA表达未见明显变化。免疫组织化学证实软骨的存在，第10天I型和II型胶原蛋白阳性，第20天仅II型胶原蛋白阳性。术后20天观察骨形成的开始。术后40天，反应性组织几乎完全变成了骨头。术后10天形成的软骨的质量和数量使这项技术潜在地用于填补大的软骨和骨缺陷。此外，骨膜骨痂的形成，为组织工程技术的研究提供了可能的有用信息。
Periosteum has chondrogenic and osteogenic potential and plays an important role in fracture healing. The purpose of this study was to evaluate the reactive tissue formed after damaging the periosteum. Damaging the periosteum may be a way to generate ectopic cartilage or bone, which may be useful for the repair of articular cartilage and bone defects. Periosteum was bilaterally dissected from the proximal medial tibia of New Zealand White rabbits. Reactive periosteal tissue was harvested 10, 20, and 40 days postsurgery and analyzed for expression of collagen types I, II, and X, aggrecan, osteopontin, and osteonectin ( by reverse transcription-polymerase chain reaction) and collagen types I and II ( by immunohistochemistry). Reactive tissue was present in 93% of cases. Histologically, this tissue consisted of hyaline cartilage at follow-up days 10 and 20. Expression of collagen type II and aggrecan was present at 10 and 20 days postsurgery. Highest expression was at 10 days. Expression of collagen type X increased up to 20 days. No significant changes in the mRNA expression of osteopontin or osteonectin were observed. Immunohistochemistry confirmed the presence of cartilage, which was positive for collagen types I and II at 10 days and only for collagen type II at 20 days. At 20 days postsurgery the onset of bone formation was also observed. At 40 days postsurgery, the reactive tissue had almost completely turned into bone. The quality and amount of cartilage formed 10 days postsurgery make this technique potentially useful to fill large cartilage and bone defects. Also, periosteal callus formation, providing possible useful information for tissue-engineering techniques, can be studied with this model.