In Vitro Visualization of Cell-to-Cell Interactions Between Natural Killer Cells and Sensory Neurons.
In Vitro Visualization of Cell-to-Cell Interactions Between Natural Killer Cells and Sensory Neurons.
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DOI：
10.1007/978-1-0716-2160-8_18
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发表时间：
2022-01-01
期刊：
影响因子：
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通讯作者：
Oh, Seog Bae
中科院分区：
文献类型：
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作者：
Kim, Hyoung Woo;Davies, Alexander J;Oh, Seog Bae
免疫系统和神经系统之间的细胞间相互作用对健康和疾病的重要性日益得到认识。细胞神经免疫相互作用的评估可以通过在体外模型系统中共同培养两个（或更多）细胞来辅助，该系统保留了神经元细胞的形态。在这里，我们描述了研究自然杀伤细胞对从同基因胚胎和成年小鼠分离的感觉神经元的细胞毒效应功能的方法。我们提出了通过活共聚焦钙成像对轴突断裂（剪枝）和动态细胞功能进行形态学分析的方法。这些技术可以很容易地适应于研究免疫细胞亚群和神经元群体的其他组合之间的相互作用。
Cell-to-cell interactions between the immune and nervous systems are increasingly recognized for their importance in health and disease. Assessment of cellular neuro-immune interactions can be aided by co-culture of two (or more) cells in an in vitro model system that preserves the morphology of neuronal cells. Here we describe methods to investigate the cytotoxic effector functions of natural killer cells on sensory neurons isolated from syngeneic embryonic and adult mice. We present methods for the morphological analysis of axon fragmentation (pruning) and dynamic cell function via live confocal calcium imaging. These techniques can easily be adapted to study interactions between other combinations of immune cell subsets and neuronal populations.