Scattering properties and femtosecond laser ablation thresholds of human and canine vocal folds at 776-nm wavelength
Scattering properties and femtosecond laser ablation thresholds of human and canine vocal folds at 776-nm wavelength
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DOI：
10.1117/1.jbo.24.8.085005
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发表时间：
2019-08-01
影响因子：
3.5
通讯作者：
Ben-Yakar, Adela
中科院分区：
文献类型：
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作者：
Andrus, Liam;Mau, Ted;Ben-Yakar, Adela
超快激光消融术可能为声带瘢痕提供一种治疗方法。在临床实施之前，必须了解VFS的光学特性，以选择合适的激光手术条件。本文报道了人和犬室颤组织上皮L(S，EP)、L(S，SLP)上皮的散射长度和消融阈值F-TH。我们的实验方法包括一种基于图像引导的激光消融方法，允许同时测定这些多层组织中的L(S)和F-TH。对8个犬标本的研究发现，L(S，EP)=75.3+/-5.7亩，L(S，SLP)=2 6.1+/-1.2亩，F-TH，F-EP=1.5 8+/-0.0 6J/cm(2)，F-TH，F-SLP=1.5 5+/-0.17J/cm(2)。通过对5例人体标本的研究，发现L(S，EP)=42.8+/-3.3µm，F-TH，F-EP=1.66+/-0.10J/cm(2)。我们研究了累积脉冲重叠对消融阈值的影响，发现除了12个重叠脉冲外，没有明显的变化。有趣的是，我们关于样品存储对猪VF散射特性的影响的研究表明，与相同的样品在等渗溶液中存储的相同样品相比，新鲜猪VF的L(S，EP)增加了60%。这些结果为临床实施VF组织选择最佳激光手术参数提供了指导。(C)作者。由SPIE在知识共享署名4.0未移植许可下发布。
Ultrafast laser ablation may provide a treatment for vocal fold (VF) scarring. Optical properties of VFs must be known prior to clinical implementation to select appropriate laser surgery conditions. We present scattering lengths of epithelium l(s,ep), superficial lamina propria l(s,SLP), and ablation thresholds F-th of human and canine VF tissues. Our experimental approach involves an image-guided, laser-ablation-based method that allows for simultaneous determination of l(s) and F-th in these multilayered tissues. Studying eight canine samples, we found l(s,ep) = 75.3 +/- 5.7 mu m, l(s,SLP) = 26.1 +/- 1.2 mu m, F-th,F-ep = 1.58 +/- 0.06 J/cm(2), and F-th,F-SLP = 1.55 +/- 0.17 J/cm(2). Studying five human samples, we found l(s,ep) = 42.8 +/- 3.3 mu m and F-th,F-ep = 1.66 +/- 0.10 J/cm(2). We studied the effects of cumulative pulse overlap on ablation threshold and found no significant variations beyond 12 overlapping pulses. Interestingly, our studies about the effect of sample storage on the scattering properties of porcine VF show a 60% increase in l(s,ep) for fresh porcine VF when compared to the same sample stored in isotonic solution. These results provide guidelines for clinical implementation by enabling selection of optimal laser surgery parameters for subsurface ablation of VF tissues. (C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License.