Induction of secondary metabolite production by fungal co-culture of Talaromyces pinophilus and Paraphaeosphaeria sp.
Induction of secondary metabolite production by fungal co-culture of Talaromyces pinophilus and Paraphaeosphaeria sp.
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DOI：
10.1007/s11418-020-01400-1
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发表时间：
2020-03-31
影响因子：
3.3
通讯作者：
Tsukamoto, Sachiko
中科院分区：
文献类型：
--
作者：
Murakami, Shunya;Hayashi, Nozomu;Tsukamoto, Sachiko
真菌共培养是一种通过激活隐藏基因来诱导次生代谢产物产生的策略。从日本宫崎县采集的土壤中分离出嗜松篮状菌和副球腔菌，在共培养过程中，我们发现了一种新的化合物他拉罗酮A（1），沿着5种已知化合物2-6。其中，青霉烷酮C（2）和D（3）的产量分别提高了27倍和6倍。基于DFT计算，3的结构应表示为具有所报道的化学位移的γ-吡啶醇形式，而不是γ-吡啶酮形式。[图形]。
Fungal co-culture is a strategy to induce the production of secondary metabolites by activating cryptic genes. We discovered the production of a new compound, talarodone A (1), along with five known compounds 2-6 in co-culture of Talaromyces pinophilus and Paraphaeosphaeria sp. isolated from soil collected in Miyazaki Prefecture, Japan. Among them, the productions of penicidones C (2) and D (3) were enhanced 27- and sixfold, respectively, by the co-culture. The structure of 3 should be represented as a gamma-pyridol form with the reported chemical shifts, but not as a gamma-pyridone form, based on DFT calculation.[GRAPHICS].