Brain natriuretic peptide is released from human astrocytoma cell line U373MG under hypoxia: a possible role in anti-apoptosis
Brain natriuretic peptide is released from human astrocytoma cell line U373MG under hypoxia: a possible role in anti-apoptosis
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DOI：
10.1677/joe-10-0230
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发表时间：
2011-01-01
影响因子：
4
通讯作者：
Okumura, Ken
中科院分区：
文献类型：
--
作者：
Katoh, Chisato;Osanai, Tomohiro;Okumura, Ken
我们最近发现急性缺血性卒中患者血浆脑型利钠肽（BNP）水平升高，但BNP的来源和作用尚不清楚。我们研究了人类星形胶质细胞在缺氧条件下是否会分泌BNP，如果分泌，涉及哪些信号通路，BNP的作用是什么。采用缺氧包埋法对人星形细胞瘤细胞株U373MG进行缺氧处理。缺氧后12 h和24 h， BNP基因表达分别增加3.9 +/- 2.5倍和6.5 +/- 2.9倍（n=6, P < 0.05）
We have recently shown that the plasma brain-type natriuretic peptide (BNP) level is elevated in acute ischemic stroke patients, but the origin and role of BNP remain unclear. We investigated whether human astrocytes secrete BNP under hypoxia, and if so, what signaling pathway is involved, and what is the role of BNP. Human astrocytoma cell line U373MG was exposed to hypoxia by Anaeropack. BNP gene expression was increased by 3.9 +/- 2.5- and 6.5 +/- 2.9-fold at 12 and 24 h after hypoxia respectively (n=6, both P