Protective Effect Against Toxoplasmosis in BALB/c Mice Vaccinated With Recombinant Toxoplasma gondii MIF, CDPK3, and 14-3-3 Protein Cocktail Vaccine.
Protective Effect Against Toxoplasmosis in BALB/c Mice Vaccinated With Recombinant Toxoplasma gondii MIF, CDPK3, and 14-3-3 Protein Cocktail Vaccine.
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接种重组弓形虫 MIF、CDPK3 和 14-3-3 蛋白混合物疫苗的 BALB/c 小鼠对弓形虫病的保护作用
DOI：
10.3389/fimmu.2021.755792
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发表时间：
2021
影响因子：
7.3
通讯作者：
Du J
中科院分区：
文献类型：
--
作者：
Liu F;Wu M;Wang J;Wen H;An R;Cai H;Yu L;Shen J;Chen L;Du J
弓形虫可以感染包括人类在内的几乎所有的内温生物，并在免疫功能低下的个体中引起危及生命的弓形虫病，从而导致严重的公共卫生问题。研制一种对付这种疾病的极好的疫苗迫在眉睫。在本研究中，我们配制了包括TgMIF、TgCDPK 3和Tg 14 -3-3蛋白的鸡尾酒蛋白疫苗，这些蛋白在T.弓形虫感染构建重组蛋白疫苗，并接种BALB/c小鼠进行免疫评价。我们将小鼠组织在疫苗的各种蛋白质组合组中，并且所有小鼠在0、2和4周时用相应的蛋白质免疫。结果表明，疫苗对小鼠T.通过细胞因子、血清抗体、脾细胞增殖试验、小鼠存活时间、虫囊负荷等指标检测弓形虫感染情况。研究表明，与其他两种蛋白组合和对照相比，用所有三种多组分蛋白疫苗免疫的小鼠引发了强烈的免疫应答，IFN-γ产生水平和IgG抗体水平最高。此外，IL-4产生和抗原特异性淋巴细胞增殖增加。与其他组小鼠相比，用三种多组分蛋白免疫的小鼠的寄生虫包囊显著减少（导致82.7%的减少），并且存活时间更长。增强的体液和细胞免疫表明，含有三种抗原的蛋白鸡尾酒疫苗对小鼠提供了有效的保护。这些结果表明，重组TgMIF，TgCDPK 3和Tg 14 -3-3多组分蛋白是潜在的候选疫苗抗弓形虫。
Toxoplasma gondii can infect almost all endotherm organisms including humans and cause life-threatening toxoplasmosis in immunocompromised individuals, which leads to serious public health problems. Developing an excellent vaccine against this disease is impending. In present study, we formulated a cocktail protein vaccine including the TgMIF, TgCDPK3, and Tg14-3-3 proteins, which play critical roles in T. gondii infection. The recombinant protein vaccines were constructed and assessed by vaccination in BALB/c mice. We organized the mice in various protein combination groups of vaccines, and all mice were immunized with corresponding proteins at 0, 2, and 4 weeks. The specific protective effects of the vaccines on mice against T. gondii were analyzed by the mensuration of cytokines, serum antibodies, splenocyte proliferation assay, survival time, and parasite cyst burden of mice after the challenge. The study indicated that mice immunized with all three multicomponent proteins vaccine triggered a strong immune response with highest levels of IFN-γ production and IgG antibody compared with the other two protein combinations and controls. Moreover, there was an increase in IL-4 production and antigen-specific lymphocyte proliferation. The parasite cysts were significantly reduced (resulting in an 82.7% reduction), and survival time was longer in immunized mice with three multicomponent proteins compared with the other groups of mice. The enhanced humoral and cell-mediated immunity indicated that the protein cocktail vaccine containing three antigens provided effective protection for mice. These results indicated that recombinant TgMIF, TgCDPK3, and Tg14-3-3 multicomponent proteins were potential candidates for vaccine against toxoplasmosis.
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影响因子：
5.7
作者：
Czarnewski P;Araújo ECB;Oliveira MC;Mineo TWP;Silva NM
通讯作者：
Silva NM
影响因子：
2.4
作者：
BUXTON, D;INNES, EA
通讯作者：
INNES, EA
DOI：
10.1016/j.bbrc.2009.01.030
发表时间：
2009-03-13
影响因子：
3.1
作者：
Richardson, Julia M.;Morrison, Lesley S.;Bland, Nicholas D.;Bruce, Sandra;Coombs, Graham H.;Mottram, Jeremy C.;Walkinshaw, Malcolm D.
通讯作者：
Walkinshaw, Malcolm D.
影响因子：
2.1
作者：
Dziadek, Bozena;Gatkowska, Justyna;Dlugonska, Henryka
通讯作者：
Dlugonska, Henryka
影响因子：
3.2
作者：
Chuang SC;Ko JC;Chen CP;Du JT;Yang CD
通讯作者：
Yang CD