Migration of Deformable Vesicles Induced by Ionic Stimuli
Migration of Deformable Vesicles Induced by Ionic Stimuli
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离子刺激诱导的可变形囊泡的迁移
DOI：
10.1021/acs.langmuir.8b02105
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发表时间：
2018
期刊：
影响因子：
3.9
通讯作者：
and Miglena I. Angelova
中科院分区：
文献类型：
--
作者：
Atsuji Kodama;Mattia Morandi;Ryuta Ebihara;Takehiro Jimbo;Masayuki Toyoda;Yuka Sakuma;Masayuki Imai;Nicolas Puff;and Miglena I. Angelova
我们研究了由 1,2-二油酰-sn-甘油-3-磷酸胆碱组成的磷脂囊泡的动力学，该囊泡由使用 CaCl2、NaCl 和 NaOH 等电解质的离子刺激触发。离子刺激诱导两种特征性囊泡动力学：由于离子与囊泡外层脂质结合而导致的变形以及由于离子浓度梯度而导致的迁移，即扩散电泳或界面能量梯度机制。我们检查了每种电解质随时间变化的变形路径，并根据表面离解模型和面积差弹性模型对其进行了分析，揭示了离子结合引起的磷脂横截面积的变化。 Ca2+和Na+等金属离子通过减小脂质的横截面积来促进向内出芽变形，而氢氧根离子(OH-)通过增加脂质的横截面积来促进向外出芽变形。当我们将这些电解质显微注射到囊泡时，观察到 CaCl2 和 NaOH 的囊泡变形和迁移之间存在很强的耦合，而对于 NaCl，这种耦合非常弱。这种差异可能源于离子的结合常数。
We have investigated the dynamics of phospholipid vesicles composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine triggered by ionic stimuli using electrolytes such as CaCl2, NaCl, and NaOH. The ionic stimuli induce two characteristic vesicle dynamics, deformation due to the ion binding to the lipids in the outer leaflet of the vesicle and migration due to the concentration gradient of ions, that is, diffusiophoresis or the interfacial energy gradient mechanism. We examined the deformation pathway for each electrolyte as a function of time and analyzed it based on the surface dissociation model and the area difference elasticity model, which reveals the change of the cross-sectional area of the phospholipid by the ion binding. The metal ions such as Ca2+and Na+encourage inward budding deformation by decreasing the cross-sectional area of a lipid, whereas the hydroxide ion (OH–) encourages outward budding deformation by increasing the cross-sectional area of a lipid. When we microinjected these electrolytes toward the vesicles, a strong coupling between the deformation and the migration of the vesicle was observed for CaCl2and NaOH, whereas for NaCl, the coupling was very weak. This difference probably originates from the binding constants of the ions.