The gastric H+,K+-ATPase is a major autoantigen in chronic Helicobacter pylori gastritis with body mucosa atrophy
The gastric H+,K+-ATPase is a major autoantigen in chronic Helicobacter pylori gastritis with body mucosa atrophy
复制标题
DOI：
10.1016/s0016-5085(98)70200-8
复制
发表时间：
1998-08-01
期刊：
影响因子：
29.4
通讯作者：
Kirchner, T
中科院分区：
文献类型：
--
作者：
Claeys, D;Faller, G;Kirchner, T
背景与目的：幽门螺杆菌感染的一个亚组患者产生抗胃壁细胞小管自身抗体。本研究的目的是确定未知的自身抗原。方法：筛选72株H. pylori感染患者、5例自身免疫性胃炎患者和36例健康对照者进行免疫组化检测小管免疫球蛋白G自身抗体。抗原特异性通过小鼠胃H+，K+-腺苷三磷酸酶（H+，K+-ATP酶）在卵母细胞中表达的免疫沉淀和人胃粘膜上的免疫印迹来确定。结果如下：在正常对照组、自身免疫性胃炎组、H+，K+-ATP酶构象肽段特异性自身抗体阳性率分别为3%（1/36）、5/5、25%（18/72）。47%（15/32）的感染者有抗小管自身抗体。未发现其他主要自身抗原。抗小管抗体和抗H+，K+-ATP酶抗体阳性的感染者胃体粘膜萎缩率为60%（9/15），而两种抗体均阴性的感染者胃体粘膜萎缩率为13%（5/37）（P < 0.01）。结论：胃H+，K+-ATP酶是H.幽门相关抗胃自身免疫因此，与经典自身免疫性胃炎密切相关的抗H+，K+-ATP酶自身抗体也是慢性H.幽门胃炎
Background & Aims: A subgroup of Helicobacter pylori-infected patients develops autoantibodies to gastric parietal cell canaliculi. The aim of this study was to define the unknown autoantigen. Methods: We screened 72 H. pylori-infected patients, 5 patients with autoimmune gastritis, and 36 healthy controls for immunoglobulin G autoantibodies to canaliculi by immunohistochemistry. The antigen specificity was determined by immunoprecipitation of the murine gastric H+,K+-adenosine triphosphatase (H+,K+-ATPase) expressed in oocytes and by immunoblotting on human gastric membranes from the body mucosa. Results: Autoantibodies specific for the conformational peptides of the H+,K+-ATPase were detected in 3% (1/36) of controls, in all patients with autoimmune gastritis (5/5), in 25% (18/72) of H. pylori-infected patients, and in 47% (15/32) of the infected patients with anticanalicular autoantibodies. No other major autoantigen was identified. Atrophy in the gastric body mucosa was found in 60% (9/15) of infected patients with both anticanalicular and anti-H+,K+-ATPase antibodies, but only in 13% (5/37) of infected patients lacking both autoantibodies (P < 0.01). Conclusions: The gastric H+,K+-ATPase is a major autoantigen in H. pylori-associated antigastric autoimmunity. Thus, anti-H+,K+-ATPase autoantibodies, which are closely linked to classical autoimmune gastritis, are also significant indicators for body mucosa atrophy in chronic H. pylori gastritis.