Expression profiling of microRNAs in optineurin (E50K) mutant transgenic mice
Expression profiling of microRNAs in optineurin (E50K) mutant transgenic mice
复制标题
optineurin (E50K) 突变转基因小鼠中 microRNA 的表达谱。
DOI：
10.3892/br.2015.565
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发表时间：
2016-02-01
影响因子：
2.3
通讯作者：
Yuan, Huiping
中科院分区：
文献类型：
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作者：
Gao, Lin;Jiang, Bo;Yuan, Huiping
转录因子视神经磷酸酶(OPTN)的E50K替换可导致原发性开角型青光眼(POAG)。为了探讨microRNAs(MiRNAs)在E50K OPTN诱导的POAG中的潜在作用，对OPTN(E50K)转基因小鼠和野生型小鼠的视网膜标本进行了miRNA表达谱分析。收集了30只转基因和30只野生型小鼠的视网膜，并使用全基因组miRNA微阵列评估了miRNA的表达。利用逆转录定量聚合酶链式反应(RT-qPCR)对OPTN(E50K)转基因小鼠视网膜组织中差异表达的miRNAs进行鉴定和验证。使用生物信息学工具进行额外的基因本体论和信号通路分析。与野生型小鼠相比，OPTN(E50K)转基因小鼠视网膜中共有48个miRNAs表达增强或降低。共有5个在OPTN(E50K)转基因小鼠中表达增加的miRNAs属于miR-8家族，可聚为一类，它们可能在OPTN(E50K)转基因小鼠的POAG发生中起调节作用。RT-qPCR结果证实，与野生型小鼠相比，OPTN(E50K)转基因小鼠视网膜中miR-141的表达显著增加。综上所述，这些结果表明某些miRNAs在OPTN(E50K)转基因小鼠的视网膜中有差异表达，并可能在OPTN(E50K)诱导的POAG的发病机制中起作用。
An E50K substitution in the transcription factor optineurin (OPTN) induces primary open-angle glaucoma (POAG). To explore the potential role of microRNAs (miRNAs) in E50K OPTN-induced POAG, miRNA expression profiling was performed on retinal samples from OPTN (E50K) transgenic and wild-type mice. The retinas were collected from 30 transgenic and 30 wild-type mice, and miRNA expression was evaluated using a genome-wide miRNA microarray. miRNAs that were differentially expressed in retinal samples from OPTN (E50K) transgenic mice were identified and validated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Additional gene ontology and signaling pathway analyses were performed using bioinformatics tools. A total of 48 miRNAs exhibited increased or decreased expression in the retinas from OPTN (E50K) transgenic mice when compared with the expression in the retinas from wild-type mice. A total of 5 miRNAs with increased expression in OPTN (E50K) transgenic mice could be grouped into one cluster as they belong to the miR-8 family and may act as regulators in the development of POAG in OPTN (E50K) transgenic mice. RT-qPCR results confirmed significantly increased expression of miR-141 in the retinas of OPTN (E50K) transgenic mice as compared to wild-type mice. In conclusion, these results show that certain miRNAs are differentially expressed in the retinas of OPTN (E50K) transgenic mice and may play roles in the pathogenesis of POAG induced by OPTN (E50K).