IMMUNOLOGICAL MEASUREMENT OF POLYCYCLIC AROMATIC HYDROCARBON-ALBUMIN ADDUCTS IN FOUNDRY WORKERS AND ROOFERS
IMMUNOLOGICAL MEASUREMENT OF POLYCYCLIC AROMATIC HYDROCARBON-ALBUMIN ADDUCTS IN FOUNDRY WORKERS AND ROOFERS
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DOI：
10.5271/sjweh.1711
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发表时间：
1991-06-01
影响因子：
6.3
通讯作者：
SANTELLA, RM
中科院分区：
文献类型：
--
作者：
LEE, BM;BAOYUN, Y;SANTELLA, RM
对高暴露于多环芳烃（PAH）的屋顶工人和铸铁工人进行了共价多环芳烃血清白蛋白加合物水平的监测，并通过敏感的竞争性酶联免疫吸附测定法对酶消化样品进行了定量分析。铸造工人的白蛋白加合物（5.22 fmol/mu-g, 0.314 mmol/mol）高于对照组（4.07 fmol/mu-g, 0.245 mmol/mol），但可能由于样本量小，仅具有临界显著性。在一部分铸造厂工人中，在休假后立即收集的样本和在工作场所暴露六周后收集的样本中观察到显着差异。屋顶工人的加合物水平（5.19 fmol/mu-g, 0.312 mmol/mol）也高于对照组（3.28 fmol/mu-g, 0.197 mmol/mol）。这些结果证明了多环芳烃蛋白加合物测量作为人类暴露于这类化学物质的标记的可行性。
Roofers and iron foundry workers with high exposure to polycyclic aromatic hydrocarbons (PAH) were monitored for levels of covalent PAH serum albumin adducts, quantitated in enzymatically digested samples by a sensitive competitive enzyme-linked immunosorbent assay. Albumin adducts were higher in the foundry workers (5.22 fmol/mu-g, 0.314 mmol/mol) than in the reference group (4.07 fmol/mu-g, 0.245 mmol/mol), but only of borderline significance probably due to the small sample size. In a subset of foundry workers, a significant difference in adduct levels was observed for samples collected immediately after vacation and after six weeks of workplace exposure. The roofers also showed higher levels of adducts (5.19 fmol/mu-g, 0.312 mmol/mol) than their reference group (3.28 fmol/mu-g, 0.197 mmol/mol). These results demonstrate the feasibility of PAH protein adduct measurement as a marker of human exposure to this class of chemicals.