Effect of progesterone on Candida albicans vaginal pathogenicity
Effect of progesterone on Candida albicans vaginal pathogenicity
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DOI：
10.1016/j.ijmm.2014.07.004
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发表时间：
2014-11-01
影响因子：
4.1
通讯作者：
Henriques, Mariana
中科院分区：
文献类型：
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作者：
Alves, Carlos Tiago;Silva, Sonia;Henriques, Mariana
白色念珠菌是大多数外阴阴道念珠菌病(VVC)病例的罪魁祸首，VVC是一种感染，主要发生在月经周期的黄体期或怀孕期间，此时孕酮水平升高。黄体酮对白念珠菌毒力的影响，即生物膜的形成和重组人阴道上皮(RHVE)的定植/入侵。通过可培养细胞计数、总质量定量和扫描电子显微镜观察生物膜的形成情况。用种属特异性多肽核酸(PNA)探针杂交荧光显微镜检测白念珠菌侵袭和定植组织的能力，并用RT-PCR念珠菌定量方法进行定量评估。用定量RT-PCR方法检测BCR1和HWP1基因在生物膜和RHVE定植细胞中的表达。结果证实，孕酮降低了白色念珠菌菌株形成生物膜以及定植和侵袭RHVE的能力。此外，研究还表明，孕酮降低了白念珠菌的重要毒力决定因素BCR1和HWP1的表达。总而言之，孕酮对白色念珠菌对阴道上皮细胞的致病性有重要影响，并可能部分解释了女性在月经周期的不同阶段对VVC的易感性。(C)2014年爱思唯尔股份有限公司。版权所有。
Candida albicans is responsible for the majority of cases of vulvovaginal candidiasis (VVC), an infection which occurs mainly during the luteal phase of the menstrual cycle or during the pregnancy, when levels of progesterone are elevated. One of the most important candidal virulence factors is the ability to adhere to host surfaces and form biofilms.The aim of this study was to determine the influence of progesterone on C. albicans virulence, namely biofilm formation and colonisation/invasion of a reconstituted human vaginal epithelium (RHVE). Biofilm formation on the RHVE was evaluated by enumeration of culturable cells, total mass quantification and scanning electron microscopy. The capacity of C. albicans strains to invade and colonise the tissue was examined by fluorescence microscopy using species-specific peptide nucleic acid (PNA) probe hybridisation, and-quantitatively evaluated by RT-PCR Candida quantification methodology. Furthermore, gene (BCR1 and HWP1) expression of biofilm and RHVE-colonising cells was evaluated by quantitative RT-PCR. Results confirmed that progesterone reduced the capacity of C albicans strains to form biofilms and to colonise and invade RHVE. Additionally, it was demonstrated that progesterone decreased expression of BCR1 and HWP1, which are important virulence determinants of C albicans. In conclusion, it was evident that progesterone can have a major influence on C albicans pathogenicity on vaginal epithelial cells and may partly explain susceptibility of women to VVC at different stages of the menstrual cycle. (C) 2014 Elsevier GmbH. All rights reserved.