Mouse testis transcriptome revealed using serial analysis of gene expression
Mouse testis transcriptome revealed using serial analysis of gene expression
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DOI：
10.1007/s00335-004-2347-7
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发表时间：
2004-06
期刊：
影响因子：
2.5
通讯作者：
Junjie Yao;T. Chiba;J. Sakai;K. Hirose;Mikio Yamamoto;A. Hada;K. Kuramoto;K. Higuchi;M. Mori
中科院分区：
文献类型：
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作者：
Junjie Yao;T. Chiba;J. Sakai;K. Hirose;Mikio Yamamoto;A. Hada;K. Kuramoto;K. Higuchi;M. Mori
我们应用基因表达系列分析（SAGE）小鼠睾丸，以揭示全球基因表达谱，并确定该谱的衰老依赖性变化。从3月龄和29月龄的BDF1小鼠和14月龄的SAMP1小鼠中获得了总共61，929个SAGE标签，包括19，323个独特标签。在睾丸中高表达的基因包括与精子发生、蛋白质代谢、能量代谢、生长和分化以及信号转导相关的基因。两个品系的老年小鼠睾丸都出现萎缩。衰老睾丸的形态学检查显示生精上皮细胞极薄，精子细胞和精母细胞数量明显减少。尽管身体退化，在老年BDF1小鼠的睾丸中，基因表达谱没有明显的总体变化。然而，在14个月大的SAMP 1小鼠中，鱼精蛋白2基因转录比BDF 1小鼠低约50%。这种减少可能与SAMP 1小鼠的少精子症和早期生殖能力下降有关。我们的SAGE结果是小鼠睾丸的第一个定量基因表达谱，并为该器官提供了可靠的转录组参考。
We applied serial analysis of gene expression (SAGE) to the mouse testis to reveal the global gene expression profile and to identify senescence-dependent changes in that profile. A total of 61,929 SAGE tags, including 19,323 unique tags, were obtained from 3- and 29-month-old BDF1 mice and 14-month-old SAMP1 mice. Genes highly expressed in the testis included those associated with spermatogenesis, protein metabolism, energy metabolism, growth and differentiation, and signal transduction. Testes from old mice of both strains appeared atrophied. Morphological examination of aged testes revealed extremely thin seminiferous epithelia and significantly decreased numbers of spermatids and spermatocytes. Despite the physical deterioration, no gross changes in the gene expression profile were apparent in the testes of old BDF1 mice. However, in 14-month-old SAMP1 mice, protamine 2 gene transcription was approximately 50% lower than in BDF1 mice. This reduction may be associated with the oligozoospermia and early decline in reproductive performance of SAMP1 mice. Our SAGE results are the first quantitative gene expression profile of the mouse testis and provide a reliable transcriptome reference for this organ.