Effects of Copper and PQQ on the Denitrification Activities of Microorganisms Facilitating Nitrite- and Nitrate-Dependent DAMO Reaction
Effects of Copper and PQQ on the Denitrification Activities of Microorganisms Facilitating Nitrite- and Nitrate-Dependent DAMO Reaction
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DOI：
10.1007/s41742-018-0118-7
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发表时间：
2018-07
影响因子：
3.2
通讯作者：
M. Hatamoto;Sho Nemoto;Takashi Yamaguchi
中科院分区：
文献类型：
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作者：
M. Hatamoto;Sho Nemoto;Takashi Yamaguchi
促进反硝化厌氧甲烷氧化（DAMO）反应的微生物生长速度缓慢；因此，有必要为这些微生物建立适当的培养条件。在本研究中，使用上流式连续培养系统评估了铜 (Cu) 和吡咯喹啉醌 (PQQ) 浓度对这些微生物亚硝酸盐和硝酸盐依赖性 DAMO 活性的影响。研究证实，6 µM Cu 和 2 nM PQQ 对亚硝酸盐依赖性 DAMO 有积极作用，但对硝酸盐依赖性 DAMO 影响不大。常规基础培养基不含PQQ；因此，在基础培养基中添加 PQQ 可能是培养促进亚硝酸盐依赖性 DAMO 的细菌的有效方法。
The growth rate of microorganisms that facilitate the denitrifying anaerobic methane oxidation (DAMO) reaction is slow; thus, establishment of appropriate cultivation conditions for these microorganisms is necessary. In this study, the effect of copper (Cu) and pyrroloquinoline quinone (PQQ) concentrations on the nitrite- and nitrate-dependent DAMO activities of these microorganisms were evaluated using an upflow-type continuous cultivation system. The study confirmed that 6 µM Cu and 2 nM PQQ had a positive effect on nitrite-dependent DAMO, but had little effect on nitrate-dependent DAMO. Conventional basal medium does not contain PQQ; therefore, supplementation of basal medium with PQQ could be an effective method for the cultivation of bacteria that facilitate nitrite-dependent DAMO.