Efficacy and safety of blue-light scleral cross-linking
Efficacy and safety of blue-light scleral cross-linking
复制标题
DOI：
10.3928/1081597x-20080901-21
复制
发表时间：
2008-09-01
影响因子：
2.4
通讯作者：
Seiler, Theo
中科院分区：
文献类型：
--
作者：
Iseli, Hans Peter;Spoerl, Eberhard;Seiler, Theo
目的：评价蓝光巩膜交联的疗效及其预防治疗巩膜下视网膜损伤的安全性。方法：使用发光二极管光源，照射面积直径为 9 mm，单侧局部用核黄素（0.5%）和蓝光（465 nm）照射赤道巩膜。治疗四周后，对动物实施安乐死，并切除暴露的巩膜和对侧眼巩膜，用于生物力学刚性和组织学视网膜细胞损伤的比较测试。进行伸长测量来评估处理后巩膜与未处理巩膜的应力-应变曲线，并对处理后巩膜和下方视网膜的光学显微镜进行比较评估。 结果：蓝光巩膜交联显示所有测试动物的应力-应变曲线硬化增加了三倍。组织学检查显示任何接受治疗的眼睛都没有视网膜损伤。结论：用核黄素和蓝光（465 nm）进行巩膜交联对巩膜具有硬化作用，但不会对视网膜造成组织学组织损伤。
PURPOSE: To evaluate the efficacy of blue-light scleral cross-linking as well as its safety in preventing retinal damage beneath the treated sclera.METHODS: Six rabbits were unilaterally treated with topical riboflavin (0.5%) and blue light (465 nm) on the equatorial sclera using a light emitting diode source with an exposure area of 9 mm in diameter. Four weeks after the treatment, the animals were euthanized and the exposed sclera and contralateral eye sclera excised for comparative testing of biomechanical rigidity and histologic retinal cellular damage. Extensiometry was performed to evaluate the stress-strain curve of treated versus untreated sclera, and light microscopy of the treated sclera and underlying retina were also comparatively evaluated.RESULTS: Blue-light scleral cross-linking showed a three-fold increased stiffening in all tested animals in the stress-strain curve. Histological investigation revealed no retinal damage in any of the treated eyes.CONCLUSIONS: Scleral cross-linking with riboflavin and blue light (465 nm) has a stiffening effect on the sclera without histological tissue damage to the retina.