Flexural rigidity of microtubules measured with the use of optical tweezers.
Flexural rigidity of microtubules measured with the use of optical tweezers.
复制标题
DOI：
--
复制
发表时间：
1996-02
影响因子：
4
通讯作者：
H. Felgner;Rainer Frank;M. Schliwa
中科院分区：
文献类型：
--
作者：
H. Felgner;Rainer Frank;M. Schliwa
使用光镊测量单个微管的弯曲刚度。提出了两种新方法。在这两个激光陷阱的光学力量被用来直接操纵微管生长的Chlamyeliumaxonemes的结束。微管的形状通过视频显微镜观察，因为当微管相对于周围的缓冲介质主动移动时，粘性流的流体动力抵消弹性恢复力。为了确定弯曲刚度，在给定的速度分布下，沿沿着的微管的弯曲进行了分析。聚合后与紫杉醇孵育的微管被测量为比未添加紫杉醇的微管更灵活。另一方面，MAP显示出增加微管刚度。
The flexural rigidity of single microtubules is measured using optical tweezers. Two new methods are presented. In both the optical forces of the laser trap are used to directly manipulate microtubules grown off the ends of Chlamydomonas axonemes. The shapes of the microtubules are observed by video microscopy as the hydrodynamic forces of viscous flow counteract the elastic restoring forces when the microtubules are moved actively relative to the surrounding buffer medium. To determine the flexural rigidity, the bending of a microtubule is analyzed under a given velocity distribution along its length. Microtubules incubated with taxol after polymerization are measured to be more flexible than those without taxol added. On the other hand, MAPs are shown to increase microtubule stiffness.