Opposite changes in the expression of clathrin and caveolin-1 in normal and cancerous human prostate tissue: putative clathrin-mediated recycling of EGFR.
Opposite changes in the expression of clathrin and caveolin-1 in normal and cancerous human prostate tissue: putative clathrin-mediated recycling of EGFR.
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DOI：
10.1007/s00418-023-02183-8
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发表时间：
2023-06
影响因子：
2.3
通讯作者：
中科院分区：
文献类型：
--
作者：
细胞内吞作用是细胞摄取大分子的重要过程，在肿瘤中被认为是一种失调现象。网格蛋白和小窝蛋白-1在受体介导的内吞作用中起主要作用。我们已经使用了一种定量的、无偏的和半自动化的方法来测量网格蛋白和小窝蛋白-1在癌性和配对的正常（癌相邻的、非癌的）人前列腺组织中的原位蛋白表达。与正常组织（N = 29，n = 67）相比，前列腺癌样品（N = 29，n = 91）中网格蛋白的表达显著（p < 0.0001）增加（N =患者数量，n =组织阵列中的核心数量）。相反，与正常前列腺组织相比，前列腺癌组织中小窝蛋白-1的表达显著降低（p < 0.0001）。这两种蛋白表达的相反变化与癌症侵袭性的增加高度相关。在前列腺癌组织中，表皮生长因子受体（EGFR）（癌发生中的关键受体）的表达与网格蛋白同时增加，表明EGFR通过网格蛋白介导的内吞作用（CME）再循环。这些结果表明，在前列腺癌中，小窝蛋白-1介导的内吞作用（CavME）可能起制动作用，CME的增加可能通过EGFR的再循环促进前列腺癌的致瘤性和侵袭性。这些蛋白质表达的变化也可能用作前列腺癌的生物标志物，以帮助诊断和预后以及临床决策。在线版本包含补充材料，可通过10.1007/s 00418 -023-02183-8获得。
Endocytosis, an important macromolecule uptake process in cells, is known to be dysregulated in cancer. Clathrin and caveolin-1 proteins play a major role in receptor-mediated endocytosis. We have used a quantitative, unbiased and semi-automated method to measure in situ protein expression of clathrin and caveolin-1 in cancerous and paired normal (cancer adjacent, non-cancerous) human prostate tissue. There was a significant (p < 0.0001) increase in the expression of clathrin in prostate cancer samples (N = 29, n = 91) compared to normal tissue (N = 29, n = 67) (N = number of patients, n = number of cores in tissue arrays). Conversely, there was a significant (p < 0.0001) decrease in expression of caveolin-1 in prostate cancer tissue compared to normal prostate tissue. The opposite change in expression of the two proteins was highly correlated to increasing cancer aggressiveness. There was also a concurrent increase in the expression of epidermal growth factor receptor (EGFR), a key receptor in carcinogenesis, with clathrin in prostate cancer tissue, indicating recycling of EGFR through clathrin-mediated endocytosis (CME). These results indicate that in prostate cancer, caveolin-1-mediated endocytosis (CavME) may be acting as a brake and increase in CME may facilitate tumorigenicity and aggressiveness of prostate cancer through recycling of EGFR. Changes in the expression of these proteins can also potentially be used as a biomarker for prostate cancer to aid in diagnosis and prognosis and clinical decision-making. The online version contains supplementary material available at 10.1007/s00418-023-02183-8.
参与前列腺癌变的分子途径：公共微阵列数据集的见解。
影响因子：
3.7
作者：
Baetke SC;Adriaens ME;Seigneuric R;Evelo CT;Eijssen LM
通讯作者：
Eijssen LM
影响因子：
3.7
作者：
Arya M;Thrasivoulou C;Henrique R;Millar M;Hamblin R;Davda R;Aare K;Masters JR;Thomson C;Muneer A;Patel HR;Ahmed A
通讯作者：
Ahmed A