Cdk1 restrains NHEJ through phosphorylation of XRCC4-like factor Xlf1.
Cdk1 restrains NHEJ through phosphorylation of XRCC4-like factor Xlf1.
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DOI：
10.1016/j.celrep.2014.11.044
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发表时间：
2014-12-24
期刊：
影响因子：
8.8
通讯作者：
Doherty AJ
中科院分区：
文献类型：
--
作者：
Hentges P;Waller H;Reis CC;Ferreira MG;Doherty AJ
Eukaryotic cells use two principal mechanisms for repairing DNA double-strand breaks (DSBs): homologous recombination (HR) and nonhomologous end-joining (NHEJ). DSB repair pathway choice is strongly regulated during the cell cycle. Cyclin-dependent kinase 1 (Cdk1) activates HR by phosphorylation of key recombination factors. However, a mechanism for regulating the NHEJ pathway has not been established. Here, we report that Xlf1, a fission yeast XLF ortholog, is a key regulator of NHEJ activity in the cell cycle. We show that Cdk1 phosphorylates residues in the C terminus of Xlf1 over the course of the cell cycle. Mutation of these residues leads to the loss of Cdk1 phosphorylation, resulting in elevated levels of NHEJ repair in vivo. Together, these data establish that Xlf1 phosphorylation by Cdc2Cdk1 provides a molecular mechanism for downregulation of NHEJ in fission yeast and indicates that XLF is a key regulator of end-joining processes in eukaryotic organisms. Cdc2Cdk1 phosphorylates the core NHEJ factor Xlf1 in fission yeast Phosphorylation of Xlf1 inhibits nonhomologous end-joining (NHEJ) Cells with phospho-null Xlf1 have elevated levels of NHEJ repair NHEJ repair can predominate over HR when Cdc2Cdk1 regulation of Xlf1 is lost Repair of DNA double-strand breaks (DSBs) by homologous recombination is activated by the cell cycle kinase Cdk1. Hentges et al. now find that the NHEJ factor Xlf1 is phosphorylated by Cdk1 and that this modification restrains end-joining in cycling cells. Removal of this regulation alters DSB pathway selection in vivo.
