Localization of a Mg2+-activated ATPase in the plasma membrane of Trypanosoma cruzi.
Localization of a Mg2+-activated ATPase in the plasma membrane of Trypanosoma cruzi.
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克氏锥虫质膜中 Mg2 激活的 ATP 酶的定位。
DOI：
10.1111/j.1550-7408.1984.tb04302.x
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
W. de Souza
中科院分区：
文献类型：
--
作者：
M. Meirelles;W. de Souza
采用Wachstein和Meisel培养培养基检测克氏锥虫表皮、球囊和血流型锥虫的atp酶活性。反应产物与覆盖细胞体和鞭毛的质膜有关，表明酶的活性。鞭毛囊内的质膜部分未见反应产物。质膜相关atp酶活性不受瓦阿因或寡霉素的抑制，在不含K+的培养液中检测到，在长时间的戊二醛固定下被抑制，当培养液中不含Mg2+时，其活性降低。采用Ernst培养基检测克氏体Na+-K+- atp酶活性。没有检测到表明该酶存在的反应产物。在克氏体中未检测到指示5′-核苷酸酶的反应产物。溶酶体检测酸性磷酸酶活性。这些结果表明，Mg2+激活的atp酶存在于克氏体的质膜中，可以作为酶标记物，在线粒体和鞭毛atp酶被抑制的情况下，评估从克氏体分离的质膜组分的纯度。
The Wachstein and Meisel incubation medium was used to detect ATPase activity in epimastigote, spheromastigote (amastigote), and bloodstream trypomastigote forms of Trypanosoma cruzi. Reaction product, indicative of enzyme activity, was associated with the plasma membrane covering the cell body and the flagellum of the parasite. No reaction product was found in the portion of the plasma membrane lining the flagellar pocket. The plasma membrane-associated ATPase activity was not inhibited by ouabain or oligomycin, was detected in incubation medium without K+, was inhibited by prolonged glutaraldehyde fixation, and its activity was diminished when Mg2+ was omitted from the incubation medium. The Ernst medium was used to detect Na+-K+-ATPase activity in T. cruzi. No reaction product indicative of the presence of this enzyme was detected. Reaction product indicative of 5'-nucleotidase was not detected in T. cruzi. Acid phosphatase activity was detected in lysosomes. Those results indicate that a Mg2+-activated ATPase is present in the plasma membrane of T. cruzi and that it can be used as an enzyme marker, provided that the mitochondrial and flagellar ATPases are inhibited, to assess the purity of plasma membrane fractions isolated from this parasite.