Applications of phasors to in vitro time-resolved fluorescence measurements.
Applications of phasors to in vitro time-resolved fluorescence measurements.
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DOI：
10.1016/j.ab.2010.11.010
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发表时间：
2011-03-01
影响因子：
2.9
通讯作者：
Jameson DM
中科院分区：
文献类型：
--
作者：
Stefl M;James NG;Ross JA;Jameson DM
处理荧光寿命数据的相量方法提供了一种简单方便的方法来表征寿命异质性并检测激发态反应的存在，例如溶剂弛豫和福斯特共振能量转移。该方法利用 M sin(Φ) 与 M cos(Φ) 的关系图，其中 M 是调制比，Φ 是从频域荧光测定中获取的相位角。相量方法的一个主要优点是它为时间分辨数据提供了一种无模型方法，并且易于目视检查。尽管相量方法最近已应用于荧光寿命成像显微镜，但尚未广泛用于比色皿研究。在本研究中，我们探索该方法在体外样品中的应用。荧光染料的二元和三元混合物的相量证明了该方法用于研究复杂混合物的实用性。还提供了激发态反应的数据，例如膜和蛋白质系统中的偶极弛豫以及 EGFP 中从色氨酸残基到发色团的能量转移。
The phasor method of treating fluorescence lifetime data provides a facile and convenient approach to characterize lifetime heterogeneity and to detect the presence of excited state reactions, such as solvent relaxation and Förster Resonance Energy Transfer. The method utilizes a plot of M sin(Φ) versus M cos(Φ), where M is the modulation ratio and Φ is the phase angle taken from frequency domain fluorometry. A principle advantage of the phasor method is that it provides a model-less approach to time-resolved data, amenable to visual inspection. Although the phasor approach has been recently applied to Fluorescence Lifetime Imaging Microscopy it has not been extensively utilized for cuvette studies. In the present study we explore the applications of the method to in vitro samples. The phasors of binary and ternary mixtures of fluorescent dyes demonstrates the utility of the method for investigating complex mixtures. Data from excited state reactions, such as dipolar relaxation in membrane and protein systems and also energy transfer from the tryptophan residue to the chromophore in EGFP, are also presented.
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影响因子：
2
作者：
Clayton, A. H. A.
通讯作者：
Clayton, A. H. A.
影响因子：
2.9
作者：
Barbieri, B;Terpetschnig, E;Jameson, DM
通讯作者：
Jameson, DM
影响因子：
3.4
作者：
Celli, A.;Sanchez, S.;Mauro, T.
通讯作者：
Mauro, T.
影响因子：
2.9
作者：
FIORINI, R;VALENTINO, M;GRATTON, E
通讯作者：
GRATTON, E
影响因子：
8
作者：
James, Nicholas G.;Ross, Justin A.;Jameson, David M.
通讯作者：
Jameson, David M.