Ontogenesis of the adrenal glucocorticoids and of the target function of the enzymatic tyrosine transaminase activity in the chick embryo.
Ontogenesis of the adrenal glucocorticoids and of the target function of the enzymatic tyrosine transaminase activity in the chick embryo.
复制标题
鸡胚中肾上腺糖皮质激素的个体发生和酶促酪氨酸转氨酶活性的目标功能。
作者：
C. Marie
肾上腺和血浆糖皮质激素测定在鸡胚发育8至19天的孵化，使用竞争性蛋白结合试验。同时，研究了肝脏中酪氨酸转氨酶活性作为肾上腺依赖性靶功能。肾上腺中糖皮质激素的总量从第11天开始增加，并在第19天达到7 ng/对腺体。这种增加与肾上腺的生长有关，因为从第9天到第18天，浓度保持恒定，约为380皮克/微克干重。第12天血浆中出现糖皮质激素，第19天其浓度升至10 ng/ml，第13天达到显著峰值。该峰未反映在酪氨酸转氨酶活性中，酪氨酸转氨酶活性的出现和发展独立于循环糖皮质激素的变化。
Adrenal and plasma glucocorticoids were determined in developing chick embryos of Gallus domesticus from days 8 to 19 of incubation, using a competitive protein-binding assay. At the same time, tyrosine transaminase activity in the liver was studied as an adrenal-dependent target function. Total amounts of glucocorticoids in the adrenal gland increased from day 11 and reached 7 ng/pair of glands on day 19. This increase was related to growth of the adrenal since concentrations remained constant at about 380 pg/micrograms dry weight from days 9 to 18. Glucocorticoids appeared in the plasma on day 12 and their concentrations rose to 10 ng/ml on day 19, with a marked peak on day 13. This peak was not reflected in the tyrosine transaminase activity which appeared and developed independently of the variations in the circulating glucocorticoids.