Sensitive immunoassays for human and rat GMFB and GMFG, tissue distribution and age-related changes
Sensitive immunoassays for human and rat GMFB and GMFG, tissue distribution and age-related changes
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DOI：
10.1016/j.bbagen.2003.12.006
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发表时间：
2004-02-24
影响因子：
3
通讯作者：
Asai, K
中科院分区：
文献类型：
--
作者：
Inagaki, M;Aoyama, M;Asai, K
我们开发了敏感和特异性的双位点酶免疫测定（EIA）的神经胶质成熟因子β（GMFB）和γ（GMFG）使用特异性抗体在兔。这些测定系统使我们能够鉴定人和大鼠样品中的GMFB和GMFG（GMFs），并且它们用于研究人和大鼠GMFs的组织分布和血清浓度。在大鼠的情况下，在中枢神经系统（脊髓除外）以及胸腺和结肠中发现相对高水平的GMFB。在胸腺、脾脏和结肠中发现较高水平的GMFG。GMFs在人体内的分布与大鼠相似。在大鼠中，GMFG的最大血清浓度是在4周龄。在出生后的前30天，两性的血肌酐水平都迅速下降。另一方面，血清中GMFB浓度随年龄变化不显著。同样，在人体中，血清中GMFG的浓度在21-30岁组中最高，并在30岁组中开始迅速下降。相反，GMFB的浓度在此期间没有显著变化。在人和大鼠中未观察到GMF血清水平的显著性别差异。目前的EIA系统对于研究人和大鼠器官中的GMF是足够灵敏的。(C)2004 Elsevier B. V.保留所有权利。
We developed sensitive and specific two-site enzyme immunoassays (EIA) for glia maturation factor beta (GMFB) and gamma (GMFG) using specific antibodies raised in rabbits. These assay systems enabled us to identify GMFB and GMFG (GMFs) in both human and rat samples and they were used to investigate the tissue distribution and serum concentrations of human and rat GMFs. In the case of rat, relatively high levels of GMFB were found in the central nervous system, except for the spinal cord, and in thymus and colon. Higher levels of GMFG were found in the thymus, spleen and colon. The distribution of GMFs in human was similar to that in rat. In the rat, the maximum serum concentration of GMFG was at 4 weeks of age. The decrease in its level was rapid for the first 30 days of life in both sexes. On the other hand, the concentration of GMFB in serum did not change significantly with age. Similarly, in human, the concentration of GMFG in serum was highest in the 21-30-year-old group and began to decrease rapidly in the 30-year-old group. In contrast, the concentration of GMFB did not change significantly during this period. No significant sex differences in the serum levels of GMFs were observed in human and rat. The'present EIA systems are sufficiently sensitive for studying GMFs in human and rat organs. (C) 2004 Elsevier B.V. All rights reserved.