FLOOR PLATE AND MOTOR-NEURON INDUCTION BY DIFFERENT CONCENTRATIONS OF THE AMINO-TERMINAL CLEAVAGE PRODUCT OF SONIC HEDGEHOG AUTOPROTEOLYSIS
FLOOR PLATE AND MOTOR-NEURON INDUCTION BY DIFFERENT CONCENTRATIONS OF THE AMINO-TERMINAL CLEAVAGE PRODUCT OF SONIC HEDGEHOG AUTOPROTEOLYSIS
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DOI：
10.1016/0092-8674(95)90397-6
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发表时间：
1995-05-05
期刊：
影响因子：
64.5
通讯作者：
JESSELL, TM
中科院分区：
文献类型：
--
作者：
ROELINK, H;PORTER, JA;JESSELL, TM
底板细胞和运动神经元的分化可以由Sonic hedgehog（SHH）诱导，所述Sonic hedgehog（SHH）是一种分泌的信号蛋白，其经历自身蛋白水解切割以产生氨基和羧基末端产物。我们发现，无论是地板细胞和运动神经元诱导的氨基末端裂解产物SHH（SHH-N）。运动神经元诱导所需的SHH-N阈值浓度比底板诱导所需的低约5倍。较高浓度的SHH-N可诱导底板细胞的分化，但以运动神经元分化为代价。我们的研究结果表明，诱导底板细胞和运动神经元的脊索在体内介导的神经板细胞暴露于不同浓度的氨基末端产物的SHH autopolytic裂解。
The differentiation of floor plate cells and motor neurons can be induced by Sonic hedgehog (SHH), a secreted signaling protein that undergoes autoproteolytic cleavage to generate amino- and carboxy-terminal products. We have found that both floor plate cells and motor neurons are induced by the aminoterminal cleavage product of SHH (SHH-N). the threshold concentration of SHH-N required for motor neuron induction is about 5-fold lower than that required for floor plate induction. Higher concentrations of SHH-N can induce floor plate cells at the expense of motor neuron differentiation. Our results suggest that the induction of floor plate cells and motor neurons by the notochord In vivo is mediated by exposure of neural plate cells to different concentrations of the aminoterminal product of SHH autoproteolytic cleavage.