The Influence of Sex, Body Mass Index, and Age on Cellular and Humoral Immune Responses Against Measles After a Third Dose of Measles-Mumps-Rubella Vaccine
The Influence of Sex, Body Mass Index, and Age on Cellular and Humoral Immune Responses Against Measles After a Third Dose of Measles-Mumps-Rubella Vaccine
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DOI：
10.1093/infdis/jiac351
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发表时间：
2022-09-19
影响因子：
6.4
通讯作者：
Kennedy, Richard B.
中科院分区：
文献类型：
--
作者：
Huy Quang Quach;Chen, Jun;Kennedy, Richard B.
在流行性腮腺炎暴发的情况下，建议接种第三剂麻疹-腮腺炎-风疹疫苗（MMR 3），但免疫应答和是否需要广泛使用MMR 3仍不确定。在此，我们在232名健康受试者的队列中表征了对MMR 3的麻疹特异性免疫应答。方法在MMR 3后第0天和第28天采集血清和外周血单个核细胞（PBMC）。麻疹特异性结合和中和抗体定量血清中的酶联免疫吸附试验和微量中和试验，分别。用灭活的麻疹病毒刺激PBMC，并通过多重测定评估细胞因子/趋化因子的释放。检查受试者的人口统计学变量与免疫结果的潜在相关性。结果研究对象中，第0天和第28天的血清学阳性率分别为95.69%和100%。抗体亲合力从第0天的38.08%显著增加至第28天的42.8%（P = .00026）。中和抗体显著增强，从第0天的928.7 mIU/mL增加到第28天的1289.64 mIU/mL（P = .0001）。同时，细胞因子/趋化因子反应基本保持不变。体重指数与炎性细胞因子/趋化因子水平显著相关。结论麻疹-腮腺炎-风疹三联疫苗（MMR 3）可增强麻疹特异性体液免疫应答，但对细胞免疫应答无明显影响。在232名接受2剂MMR免疫接种的健康受试者队列中进行麻疹特异性研究，支持将其用于血清阴性或高风险个体。
Background A third dose of measles-mumps-rubella vaccine (MMR3) is recommended in mumps outbreak scenarios, but the immune response and the need for widespread use of MMR3 remain uncertain. Herein, we characterized measles-specific immune responses to MMR3 in a cohort of 232 healthy subjects. Methods Serum and peripheral blood mononuclear cells (PBMCs) were sampled at day 0 and day 28 after MMR3. Measles-specific binding and neutralizing antibodies were quantified in sera by enzyme-linked immunosorbent assay and a microneutralization assay, respectively. PBMCs were stimulated with inactivated measles virus, and the release of cytokines/chemokines was assessed by a multiplex assay. Demographic variables of subjects were examined for potential correlations with immune outcomes. Results Of the study participants, 95.69% and 100% were seropositive at day 0 and day 28, respectively. Antibody avidity significantly increased from 38.08% at day 0 to 42.8% at day 28 (P = .00026). Neutralizing antibodies were significantly enhanced, from 928.7 at day 0 to 1289.64 mIU/mL at day 28 (P = .0001). Meanwhile, cytokine/chemokine responses remained largely unchanged. Body mass index was significantly correlated with the levels of inflammatory cytokines/chemokines. Conclusions Measles-specific humoral immune responses, but not cellular responses, were enhanced after MMR3 receipt, extending current understanding of immune responses to MMR3 and supporting MMR3 administration to seronegative or high-risk individuals.A third dose of measles-mumps-rubella vaccine (MMR) boosted humoral immune responses, but not cellular immune responses, specific to measles in a cohort of 232 healthy subjects immunized with 2 doses of MMR, supporting its administration to seronegative or high-risk individuals.