The complete mitochondrial genome sequence of the hydrothermal vent galatheid crab Shinkaia crosnieri (Crustacea: Decapoda: Anomura): a novel arrangement and incomplete tRNA suite.
The complete mitochondrial genome sequence of the hydrothermal vent galatheid crab Shinkaia crosnieri (Crustacea: Decapoda: Anomura): a novel arrangement and incomplete tRNA suite.
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DOI：
10.1186/1471-2164-9-257
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发表时间：
2008-05-30
期刊：
影响因子：
4.4
通讯作者：
Yang WJ
中科院分区：
文献类型：
--
作者：
Yang JS;Nagasawa H;Fujiwara Y;Tsuchida S;Yang WJ
后生动物线粒体基因组通常由相同的37个基因组成。这些基因包含有用的信息系统发育分析和进化建模。虽然迄今已确定了1 000多种动物的完整线粒体基因组，但由于收集的标本稀少，迄今为止热液喷口物种仍被排除在外。热液喷口盔状蟹Shinkaia crosnieri的线粒体基因组长15，182 bp，由13个蛋白质编码基因、2个核糖体RNA基因和18个转运RNA基因组成。基因组的总AT含量为72.9%，这是十足类动物的典型特征。根据其位置和AT丰富度，我们确定了一个327 bp的非编码控制区。这是迄今为止在甲壳类动物中发现的最小的控制区域。解决了细胞质tRNA输入的机制，以补偿四个缺失的tRNA。色葡萄crosnieri mitogenome展示了线粒体基因的新排列。我们调查了线粒体基因序列，发现至少有六个重排的祖先泛甲壳动物（甲壳动物+六足动物）模式先后发生。密码子使用、核苷酸组成和偏好性与其它十足类动物无显著差异。系统发育分析，使用串联的核苷酸和氨基酸序列的13个蛋白质编码基因证明与以前的分类的基础上，他们的形态是一致的。本研究将为热液喷口生态系统的基因组和进化研究提供大量有用数据。十足类的线粒体遗传特征在这种情况下，S。crosnieri，尽管缺乏几个tRNA和一些戏剧性的重排。我们的研究结果可能为喷口物种起源的移民假说提供证据。
Metazoan mitochondrial genomes usually consist of the same 37 genes. Such genes contain useful information for phylogenetic analyses and evolution modelling. Although complete mitochondrial genomes have been determined for over 1,000 animals to date, hydrothermal vent species have, thus far, remained excluded due to the scarcity of collected specimens. The mitochondrial genome of the hydrothermal vent galatheid crab Shinkaia crosnieri is 15,182 bp in length, and is composed of 13 protein-coding genes, two ribosomal RNA genes and only 18 transfer RNA genes. The total AT content of the genome, as is typical for decapods, is 72.9%. We identified a non-coding control region of 327 bp according to its location and AT-richness. This is the smallest control region discovered in crustaceans so far. A mechanism of cytoplasmic tRNA import was addressed to compensate for the four missing tRNAs. The S. crosnieri mitogenome exhibits a novel arrangement of mitochondrial genes. We investigated the mitochondrial gene orders and found that at least six rearrangements from the ancestral pancrustacean (crustacean + hexapod) pattern have happened successively. The codon usage, nucleotide composition and bias show no substantial difference with other decapods. Phylogenetic analyses using the concatenated nucleotide and amino acid sequences of the 13 protein-coding genes prove consistent with the previous classification based upon their morphology. The present study will supply considerable data of use for both genomic and evolutionary research on hydrothermal vent ecosystems. The mitochondrial genetic characteristics of decapods are sustained in this case of S. crosnieri despite the absence of several tRNAs and a number of dramatic rearrangements. Our results may provide evidence for the immigrating hypothesis about how vent species originate.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
2.5
作者：
Lohse, Marc;Drechsel, Oliver;Bock, Ralph
通讯作者：
Bock, Ralph
影响因子：
3.5
作者：
Ivey, Jennifer L.;Santos, Scott R.
通讯作者：
Santos, Scott R.
DOI：
10.1073/pnas.0400941101
发表时间：
2004-07-20
影响因子：
11.1
作者：
Helfenbein, KG;Fourcade, HM;Boore, JL
通讯作者：
Boore, JL