Active oxygen processing for acrylic intraocular lenses to prevent posterior capsule opacification
Active oxygen processing for acrylic intraocular lenses to prevent posterior capsule opacification
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DOI：
10.1016/j.jcrs.2006.02.042
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发表时间：
2006-06-01
影响因子：
2.8
通讯作者：
Obara, Yoshitaka
中科院分区：
文献类型：
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作者：
Matsushima, Hiroyuki;Iwamoto, Hidetoshi;Obara, Yoshitaka
目得：目的：评价活性氧处理丙烯酸人工晶状体（Acrylic intraocular lenses，IOL）表面预防继发性后囊膜混浊（posterior capsule opacification，PCO）的效果。对IOL表面进行元素分析（化学分析电子光谱[ESCA]），以确认表面改性。UV/O-3或氩等离子体处理产生的变化进行了检查纤维连接蛋白和透镜上皮细胞（LEC）的粘附。为了评价经处理的IOL对PCO的预防作用，使用了8周龄白化病兔。随机对兔眼进行超声乳化并植入3种不同的IOL：UV/O-3处理的IOL、氩等离子体处理的IOL和对照IOL。2周后，处死家兔，解剖其球体并用10%甲醛固定。结果：经ESCA分析，人工晶状体表面含氮量增加，羟基和羧基取代基团增多，而羟基和羧基取代基团增多。UV/O-3处理和氩等离子体处理的样品上的纤连蛋白粘附和LEC粘附增加。在未处理组中，有统计学意义上的抑制PCO的形成在UV/O-3处理和氩等离子体处理groups.CONCLUSION：活性氧处理和氩等离子体照射的人工晶状体表面是有效的，在防止继发性PCO白内障手术后。
PURPOSE: To evaluate active oxygen processing on the surface of acrylic intraocular lenses (IOLs) to prevent secondary posterior capsule opacification (PCO).SETTING: Department of Ophthalmology, Dokkyo Medical University School of Medicine, Mibu City, Tochigi, Japan.METHODS: Acrylic IOLs were prepared, and ultraviolet (UV)/ozone (UV/O-3) or argon plasma was irradiated to the surface of the IOLs. Elemental analysis (electron spectroscopy for chemical analysis [ESCA]) of the IOL surfaces was performed to confirm surface modification. Changes produced by UV/O-3 or argon plasma treatment were examined for fibronectin and lens epithelial cell (LEC) adhesion. To evaluate the PCO prevention by treated IOLs, 8-week-old albino rabbits were used. The rabbit eyes randomly had phacoemulcification and implantation of 3 different IOLs: the UV/O-3-treated IOLs, argon plasma-treated IOLs, and the control IOLs. After 2 weeks, the rabbits were killed and their globes were dissected and fixed using formaldehyde 10%. The PCO was observed using light microscopy (DX51, ORIMPUS) after hematoxylin and eosin staining.RESULTS: Comparison of IOL surface composition by ESCA showed an increase in nitrogen content and hydroxyl substitute and carboxyl substitute groups on surfaces of treated IOLs. The fibronectin adhesion and the LEC adhesion on the UV/O-3-treated and argon plasma-treated samples were increased. In the untreated group, there was statistically significant inhibition of PCO formation in the UV/O-3-treated and argon plasma-treated groups.CONCLUSION: Active oxygen processing and argon plasma irradiation on the surface of IOLs was effective in preventing secondary PCO after cataract surgery.