Hierarchical Microspheres Constructed from Chitin Nanofibers Penetrated Hydroxyapatite Crystals for Bone Regeneration
Hierarchical Microspheres Constructed from Chitin Nanofibers Penetrated Hydroxyapatite Crystals for Bone Regeneration
复制标题
甲壳素纳米纤维穿透羟基磷灰石晶体构建的分层微球用于骨再生
DOI：
10.1021/acs.biomac.7b00408
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发表时间：
2017-07-01
影响因子：
6.2
通讯作者：
Zhang, Lina
中科院分区：
文献类型：
--
作者：
Duan, Bo;Shou, Kangquan;Zhang, Lina
几丁质作为矿物质模板大量存在于蟹壳和虾壳中，启发我们将其矿化，用于制作植骨材料。本研究以几丁质纳米纤维微球为基体，原位合成羟基磷灰石（HA）晶体，包括微片、亚微米针和亚微米球，这些晶体被长几丁质纳米纤维穿透，形成层次结构。的形状和大小。通过改变透明质酸的合成工艺，可以控制透明质酸晶体的形成。甲壳素与透明质酸的界面粘附紧密。通过在复合微球中形成非共价键，使得羟基苯胺均匀地分散并结合在甲壳素纳米纤维上。在我们的研究中，几丁质和透明质酸的内在生物相容性有助于骨细胞的粘附和骨传导。此外，亚微米针和亚微米球透明质酸晶体的几丁质微球显著促进体外细胞粘附和体内骨愈合。实验证明，由于复合微球独特的表面微观结构和生物相容性，在无生长因子和无细胞状态下，具有1.5 cm半径缺陷的兔子在三个月内几乎完全治愈。微球支架具有良好的生物功能和良好的生物降解性。本研究为构建天然高分子基有机无机杂化骨再生微球开辟了一条新途径。
Chitin exists abundantly in crab and shrimp shells as the template of the minerals, which inspired us to mineralize it for fabricating bone grafting materials. In the present work, chitin nanofibrous microspheres were used as the matrix for in situ synthesis of hydroxyapatite (HA) crystals including microflakes, submicron-needles, and submicron-spheres, which were penetrated by long chitin nanofibers, leading to the hierarchical structure. The shape and size of the. HA crystals could be controlled by changing the HA synthesis process. The tight interface adhesion between chitin and HA. through the noncovanlent bonds occurred in the composite microspheres, and HAs were homogeneously dispersed and bounded to the chitin nanofibers. In our findings, the inherent biocompatibilities of the both chitin and HA contributed the bone cell adhesion and osteoconduction. Moreover, the chitin microsphere with submicron-needle and submicron-sphere HA crystals remarkably promoted in vitro cell adhesion and in vivo bone healing. It was demonstrated that rabbits with 1.5 cm radius defect were almost cured completely within three months in a growth factor- and cell-free state, as a result of the unique surface microstructure and biocompatibilities of the composite microspheres. The microsphere scaffold displayed excellent biofunctions and an appropriate biodegradability. This work opened up a new avenue to construct natural polymer-based organic inorganic hybrid microspheres for bone regeneration.