Dietary intervention with narrow-leaved cattail rhizome flour (Typha angustifolia L.) prevents intestinal inflammation in the trinitrobenzenesulphonic acid model of rat colitis.
Dietary intervention with narrow-leaved cattail rhizome flour (Typha angustifolia L.) prevents intestinal inflammation in the trinitrobenzenesulphonic acid model of rat colitis.
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DOI：
10.1186/1472-6882-12-62
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发表时间：
2012-05-04
影响因子：
--
通讯作者：
Di Stasi LC
中科院分区：
文献类型：
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作者：
Fruet AC;Seito LN;Rall VL;Di Stasi LC
炎症性肠病（IBD）是由肠道免疫系统、氧化应激和对管腔微生物群的耐受性丧失驱动的肠上皮的慢性炎症。使用含有诸如纤维和碳水化合物和/或抗氧化剂化合物的成分的膳食产品已被用作肠道疾病的治疗策略，因为这些产品被认为在免疫系统和结肠微生物群的调节中是有效的。研究了香蒲粉（TyphaangustifoliaL.）在大鼠结肠炎的三硝基苯磺酸（TNBS）模型中。此外，我们研究了香蒲根茎粉对泼尼松龙的肠道抗炎活性的影响，泼尼松龙是用于治疗人类IBD的参考药物。本研究包括香蒲（TyphaangustifoliaL.）根茎制粉;评价香蒲根茎的定性植物化学特征;评价香蒲根茎粉在TNBS诱导的大鼠结肠炎中的功效;评价香蒲根茎粉对泼尼松龙的肠抗炎活性的协同作用;宏观的、临床的、生化的组织病理学和微生物学研究，以评估香蒲根茎粉的愈合作用及其在TNBS诱导的大鼠结肠炎中的协同作用。资料采用方差分析、Kruskal-Wallis检验和χ2检验。我们测试了几种浓度的香蒲粉，发现膳食补充10%香蒲粉在减少病变的扩展、结肠重量比、与邻近器官的粘连和腹泻方面显示出最佳效果。这些作用与抑制髓过氧化物酶（MPO）和碱性磷酸酶（AP）的活动和谷胱甘肽（GSH）耗竭的衰减。10%香蒲粉与泼尼松龙作用相当，无协同作用。根茎粉中检测到皂苷、黄酮和香豆素。在日粮中添加香蒲粉后，乳酸菌总数没有变化。在大鼠日粮中添加10%香蒲粉及其与泼尼松龙的组合预防TNBS诱导的结肠损伤，但未观察到协同作用。TNBS诱导的结肠损伤的预防与肠道氧化应激的改善有关，这可能是由于香蒲根茎中检测到的活性化合物的抗氧化特性。这种保护作用与乳酸菌计数的改善无关。
Inflammatory bowel disease (IBD) is a chronic inflammation of the intestinal epithelium that is driven by the intestinal immune system, oxidative stress and the loss of tolerance to the luminal microbiota. The use of dietary products containing ingredients such as fibres and carbohydrates and/or antioxidant compounds have been used as a therapeutic strategy for intestinal diseases because these products are considered effective in the modulation of the immune system and colonic microbiota. We investigated the beneficial effects of cattail rhizome flour (Typha angustifolia L.) in the trinitrobenzenesulphonic acid (TNBS) model of rat colitis. In addition, we investigated the effects of cattail rhizome flour on the intestinal anti-inflammatory activity of prednisolone, which is a reference drug that is used for treatment of human IBD. The present study included the preparation of flour from rhizomes of cattail (Typha angustifolia L.); an evaluation of the qualitative phytochemical profile of cattail rhizomes; an evaluation of the efficacy of cattail rhizome flour in TNBS-induced rat colitis; an evaluation of the synergistic effects of cattail rhizome flour on the intestinal anti-inflammatory activity of prednisolone; and macroscopic, clinical, biochemical, histopathological and microbiological studies to assess the healing effects of cattail rhizome flour and its synergistic effects in TNBS-induced rat colitis. The data were analysed by ANOVA, Kruskal-Wallis and χ2 tests. We tested several concentrations of cattail rhizome flour and found that dietary supplementation with 10% cattail rhizome flour showed the best effects at reducing the extension of the lesion, the colon weight ratio, adherences to adjacent organs and diarrhoea. These effects were related to inhibition of myeloperoxidase (MPO) and alkaline phosphatase (AP) activities and an attenuation of glutathione (GSH) depletion. The 10% cattail rhizome flour was as effective as prednisolone, and no synergistic effects were observed. Saponins, flavonoids and coumarins were detected in the rhizome flour. No changes were observed in the total number of lactic bacteria after dietary supplementation with cattail rhizome flour. Dietary supplementation with 10% cattail rhizome flour and its combination with prednisolone prevent TNBS-induced colonic damage in rats, but no synergistic effects were observed. The prevention of TNBS-induced colon damage was associated with an improvement in intestinal oxidative stress, which likely resulted from the antioxidant properties of the active compounds detected in the cattail rhizome. This protective effect was not related to an improvement in lactic bacteria counts.