Similarities between heterophilic and homophilic cadherin adhesion
Similarities between heterophilic and homophilic cadherin adhesion
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DOI：
10.1073/pnas.0606701103
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发表时间：
2006-10-17
影响因子：
11.1
通讯作者：
Leckband, D. E.
中科院分区：
文献类型：
--
作者：
Prakasam, A. K.;Maruthamuthu, V.;Leckband, D. E.
在发育过程中将细胞分离成组织特异性亚群的机制主要归因于细胞间粘附的差异。这一过程需要钙依赖性糖蛋白的钙粘蛋白家族。一个广泛持有的观点是，细胞表面不同钙粘蛋白之间的蛋白质水平区分驱动了这一分选过程。尽管这种假设的分子选择性，粘附选择性尚未在蛋白质水平上得到定量验证。在这项工作中，分子力测量和珠聚集测定测试是否在钙粘蛋白键强度的差异可以解释细胞分选在体内和体外。用鸡N-钙粘蛋白、犬E-钙粘蛋白和非洲爪蟾C-钙粘蛋白进行研究。定性珠聚集和定量力测量都表明钙粘蛋白交叉反应。此外，嗜异性粘附并不比嗜同性粘附弱，并且测量的粘附差异与细胞分选行为无关。这些结果表明，在形态发生过程中细胞分离的基础并不完全映射到钙粘蛋白粘附的蛋白质水平的差异。
The mechanism that drives the segregation of cells into tissue-specific subpopulations during development is largely attributed to differences in intercellular adhesion. This process requires the cadherin family of calcium-dependent glycoproteins. A widely held view is that protein-level discrimination between different cadherins on cell surfaces drives this sorting process. Despite this postulated molecular selectivity, adhesion selectivity has not been quantitatively verified at the protein level. In this work, molecular force measurements and bead aggregation assays tested whether differences in cadherin bond strengths could account for cell sorting in vivo and in vitro. Studies were conducted with chicken N-cadherin, canine E-cadherin, and Xenopus C-cadherin. Both qualitative bead aggregation and quantitative force measurements show that the cadherins cross-react. Furthermore, heterophilic adhesion is not substantially weaker than homophilic adhesion, and the measured differences in adhesion do not correlate with cell sorting behavior. These results suggest that the basis for cell segregation during morphogenesis does not map exclusively to protein-level differences in cadherin adhesion.