Classification of gas5 as a multi-small-nucleolar-RNA (snoRNA) host gene and a member of the 5′-terminal oligopyrimidine gene family reveals common features of snoRNA host genes
Classification of gas5 as a multi-small-nucleolar-RNA (snoRNA) host gene and a member of the 5′-terminal oligopyrimidine gene family reveals common features of snoRNA host genes
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DOI：
10.1128/mcb.18.12.6897
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发表时间：
1998-12-01
影响因子：
5.3
通讯作者：
Steitz, JA
中科院分区：
文献类型：
--
作者：
Smith, CM;Steitz, JA
我们已经确定了气体5（生长停滞特异性转录5）作为一个非蛋白质编码的多个小核仁RNA（snoRNA）的主机基因类似UHG（U22主机基因）。在小鼠gad基因的11个内含子内编码的是9个（在人中为10个）盒CID snoRNA，预测其在rRNA的2 ′-O-甲基化中起作用。小鼠和人gad基因之间唯一的保守区域是它们的snoRNA和5 '端序列。对小鼠gas 5转录物的5'端作图表明，它具有5'端寡嘧啶（5 'TOP）类基因的寡嘧啶段特征。放线菌酮、帕他霉素或雷帕霉素（它们特异性抑制5 'TOP mRNA的翻译）抑制细胞生长或抑制翻译，导致gad剪接RNA的积累。将gas 5归类为5 'TOP基因为为什么它是生长停滞特异性转录物提供了解释：虽然剪接的gas 5 RNA通常与核糖体相关并迅速降解，但在细胞生长停滞期间，它在mRNP颗粒中积累，如已报道的其他5' TOP信息。引人注目的是，对目前已知的snoRNA宿主基因转录物的5'端序列的检查揭示，它们都表现出5' TOP基因家族的特征。
We have identified gas5 (growth arrest-specific transcript 5) as a non-protein-coding multiple small nucleolar RNA (snoRNA) host gene similar to UHG (U22 host gene). Encoded within the 11 introns of the mouse gad gene are nine (10 in human) box CID snoRNAs predicted to function in the 2'-O-methylation of rRNA. The only regions of conservation between mouse and human gad genes are their snoRNAs and 5'-end sequences. Mapping the 5' end of the mouse gas5 transcript demonstrates that it possesses an oligopyrimidine tract characteristic of the 5'-terminal oligopyrimidine (5'TOP) class of genes. Arrest of cell growth or inhibition of translation by cycloheximide, pactamycin, or rapamycin-which specifically inhibits the translation of 5'TOP mRNAs-results in accumulation of the gad spliced RNA. Classification of gas5 as a 5'TOP gene provides an explanation for why it is a growth arrest specific transcript: while the spliced gas5 RNA is normally associated with ribosomes and rapidly degraded, during arrested cell growth it accumulates in mRNP particles, as has been reported for other 5'TOP messages. Strikingly, inspection of the 5' end sequences of currently known snoRNA host gene transcripts reveals that they all exhibit features of the 5'TOP gene family.