Needle-based confocal endomicroscopy for in vivo histology of intra-abdominal organs: first results in a porcine model (with videos)
Needle-based confocal endomicroscopy for in vivo histology of intra-abdominal organs: first results in a porcine model (with videos)
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DOI：
10.1016/j.gie.2010.01.010
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发表时间：
2010-06-01
影响因子：
7.7
通讯作者：
Meining, Alexander
中科院分区：
文献类型：
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作者：
Becker, Valentin;Wallace, Michael B.;Meining, Alexander
背景：基于探针的共聚焦激光扫描显微内镜能够在体内对粘膜层进行真实的组织病理学检查。最近，一种新的共聚焦微型探针的原型已经被开发出来，这种探针足够小，可以通过22号穿刺针引入。目的：本研究的目的是评估这种基于针的共聚焦激光扫描显微内镜（nCLE）用于猪模型中各种器官的体内组织学的可行性。设计：可行性研究。设置：非生存动物实验的动物全身麻醉下在三个学术centers.Interventions：10只猪进行了检查，而他们全身麻醉。使用EUS引导的器官穿刺或自然孔口经腔内镜手术（NOTES）程序。通过22号针头插入共聚焦微型探针，在静脉注射荧光素（5-10 mL 1%或2 mL 10%溶液）后，对各种腹腔内结构和器官（淋巴结、隔膜、卵巢、肝脏、脾脏和胰腺）进行穿刺。记录实时序列。活检标本采取标准的组织病理学。主要结果测量和结果：这是技术上可行的，引入针为基础的共聚焦微型探针到各种器官的时候，EUS或NOTES程序。该设备使实时在体内收集的图像在组织学分辨率和可接受的图像质量从几个腹内器官interrogated.Limitations：数据进行了评估，在实验动物设置和健康organsonlines.Conclusions：针为基础的共聚焦激光显微内镜（CLE）的腹内器官在猪模型是可行的。这种创新的微创技术有可能在EUS穿刺或NOTES腹腔镜检查期间促进体内组织学检查。（Gastrointest Enclose 2010;71：1260-6.）
Background: Probe-based confocal laser scanning endomicroscopy enables in vivo real time histopathology of the mucosa layer. Recently, a prototype of a new confocal miniprobe has been developed that is small enough to be introduced through a 22-gauge puncture needle.Objective: The aim of this study was to evaluate the feasibility of such needle-based confocal laser scanning endomicroscopy (nCLE) for in vivo histology of various organs in a porcine model.Design: Feasibility study.Setting: Nonsurvival animal experiments with the animals under general anesthesia at three academic centers.Interventions: Ten pigs were examined while they were under general anesthesia. Either EUS-guided organ puncture or natural-orifice transluminal endoscopic surgery (NOTES) procedure was used. The confocal miniprobe was inserted through the 22-gauge needle, and puncture of various intra-abdominal structures and organs was performed (lymph nodes, diaphragm, ovaries, liver, spleen, and pancreas) after intravenous injection of fluorescein (5-10 mL 1% or 2 mL 10% solution). Real-time sequences were recorded. Biopsy specimens were taken for standard histopathology.Main Outcome Measurements and Results: It was technically feasible to introduce the needle-based confocal miniprobe into various organs at the time of EUS or NOTES procedures. The device enabled real-time in vivo collection of images at histologic resolutions and of acceptable image quality from several intra-abdominal organs interrogated.Limitations: Data were assessed in an experimental animal setting and on healthy organs only.Conclusions: Needle-based confocal laser endomicroscopy (CLE) of intra-abdominal organs is feasible in a porcine model. This innovative, minimally invasive technique has the potential to facilitate in vivo histology during EUS punctures or NOTES peritoneoscopy. (Gastrointest Enclose 2010;71:1260-6.)