Glucose starvation reduces IGF-I mRNA in tumor cells: evidence for an effect on mRNA stability.
Glucose starvation reduces IGF-I mRNA in tumor cells: evidence for an effect on mRNA stability.
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葡萄糖饥饿会减少肿瘤细胞中的 IGF-I mRNA：影响 mRNA 稳定性的证据。
DOI：
10.1006/bbrc.2000.2286
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Adamo,ML
中科院分区：
文献类型：
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作者：
Wang,L;Yang,H;Adamo,ML
The purpose of this study was to characterize the mechanisms by which glucose regulates IGF-I gene expression in rat C6 glioma cells and in rat GH3 pituitary adenoma cells. Glucose starvation for periods of 12 to 48 h decreased IGF-I mRNA levels. In contrast, there was no stimulation of IGF-I mRNA by medium glucose between 1 and 25 mM over a 24-h period. Studies with hexoses and glycolytic metabolites suggested that glucose metabolism was required to maintain IGF-I mRNA. Glucose starvation lowered IGF-I mRNA half-life in both C6 and GH3 cells. Protein synthesis inhibition lowered IGF-I mRNA by about 20% in glucose-fed C6 and GH3 cells, while potently increasing IGF-I mRNA in glucose-starved C6 cells and not altering IGF-I mRNA in glucose-starved GH3 cells. Our results suggest that in these tumor cells, IGF-I mRNA stability is reduced by glucose starvation, secondary to a deficiency in intracellular glucose metabolism. Ongoing protein synthesis is not required for this mRNA de-stabilizing effect in GH3 cells. Rather, in glucose-starved C6 cells, decreased IGF-I mRNA stability may result from the action of a labile protein.
