The mycorrhizal community of the epiphytic orchid Thrixspermum japonicum is strongly biased toward a single Ceratobasidiaceae fungus, despite a wide range of fungal partners
The mycorrhizal community of the epiphytic orchid Thrixspermum japonicum is strongly biased toward a single Ceratobasidiaceae fungus, despite a wide range of fungal partners
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附生兰花 Thrixspermum japonicum 的菌根群落强烈偏向于单一的角担子科真菌，尽管有广泛的真菌伙伴
DOI：
10.1002/ajb2.1575
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发表时间：
2020
影响因子：
3
通讯作者：
Ogura‐Tsujita Yuki
中科院分区：
文献类型：
--
作者：
Rammitsu Kento;Yukawa Tomohisa;Yamashita Yumi;Isshiki Shiro;Ogura‐Tsujita Yuki
兰花主要依靠菌根真菌在整个生命周期中获得营养。附生兰花占兰花多样性的69%。在其树栖栖息地的水和养分的不稳定的可用性往往导致严重的水和养分压力。因此，菌根协会可能是重要的附生兰花的生存，但我们的理解仍然有限。在这里，我们调查了菌根社区在一个单一的附生兰花物种，使用更多的样品比在任何以前的study.METHODS我们评估的菌根群落Thrixspermum japonicum，在日本的温带地区最常见的附生兰花之一。在1300 km范围内的20个地点，从28个寄主树种中收集了144个个体。根据核核糖体DNA内转录间隔区序列对这些菌根真菌进行了鉴定，并根据97%的序列相似性分配了操作分类单位（OTU）。这些OTU广泛分布在两个真菌科的系统发育树中。然而，一个单一的Ceratobasidiaceae OTU占所有真菌序列的49.7%，是占主导地位的样品从15个寄主树种和12 sites.CONCLUSIONSOUR的结果意味着，尽管有广泛的菌根合作伙伴，T. japonicum主要与一个单一的真菌类群在大多数的网站之间的寄主树种调查。这些发现有助于阐明真菌在附生生境中的共生关系。
PREMISEOrchids depend primarily on mycorrhizal fungi to obtain nutrients throughout their life cycle. Epiphytic orchids account for 69% of orchid diversity. The unstable availability of water and nutrients in their arboreal habitats often results in severe water and nutrient stresses. Consequently, mycorrhizal associations may be important for the survival of epiphytic orchids, but our understanding thereof remains limited. Here, we investigated the mycorrhizal community in a single epiphytic orchid species, using more samples than in any previous study.METHODSWe assessed the mycorrhizal communities ofThrixspermum japonicum, one of the most common epiphytic orchids in the temperate region of Japan. In total, 144 individuals were collected from 28 host tree species at 20 sites across 1300 km. The mycorrhizal fungi were identified based on nuclear ribosomal DNA internal transcribed spacer sequences and assigned operational taxonomic units (OTUs) based on 97% sequence similarity.RESULTSWe obtained 24 OTUs; 9 belonged to the Ceratobasidiaceae and 15 to the Tulasnellaceae. These OTUs are widely distributed throughout the phylogenetic trees of the two fungal families. However, a single Ceratobasidiaceae OTU accounted for 49.7% of all fungal sequences and was predominant in samples from 15 host tree species and 12 sites.CONCLUSIONSOur results imply that despite having a broad range of mycorrhizal partners,T.japonicumwas predominantly associated with a single fungal taxon at most of the sites among the host‐tree species investigated. These findings contribute to elucidating mycorrhizal symbiosis in epiphytic habitats.