Survivin expression in tumor cell nuclei is predictive of a favorable prognosis in gastric cancer patients
Survivin expression in tumor cell nuclei is predictive of a favorable prognosis in gastric cancer patients
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DOI：
10.1016/s0304-3835(00)00677-7
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发表时间：
2001-02-10
期刊：
影响因子：
9.7
通讯作者：
Takano, Y
中科院分区：
文献类型：
--
作者：
Okada, E;Murai, Y;Takano, Y
为探讨Survivin在胃癌细胞中的生物学作用，应用一种商品化的抗Survivin抗体(SURV11A)，对133例胃癌组织进行了免疫组织化学研究。应用逆转录聚合酶链式反应(RT-PCR)、Southern印迹、Western blotting和免疫荧光等方法对5株胃癌细胞株Survivin的定位进行了研究。胃癌细胞Survivin核染色阳性者109例(82.0%)，多因素分析显示Survivin核阳性者预后良好。Survivin核阳性也与较年轻的年龄和较低的脉管癌侵袭率相关。而Survivin胞浆阳性者117例(88.0%)，与肿瘤的进展及预后无关。结果表明，Survivin存在于大多数胃癌细胞中，但核定位可能在阻碍肿瘤进展中发挥重要的生理作用。(C)2001爱思唯尔爱尔兰科学有限公司。保留所有权利。
One hundred and thirty three gastric cancer cases were investigated immunohistochemically to clarify the biological role of survivin in gastric cancer cells using a commercially available anti-survivin antibody (SURV11A). Five gastric cancer cell lines were employed to assess localization of survivin by reverse-transcription-polymerase chain reaction (RT-PCR) Southern blotting, Western blotting and immunofluorescence, signals being found in both nucleus and cytoplasm. Survivin nuclear staining of gastric cancer cells was evident in 109 of 133 cases (82.0%) and associated with a favorable prognosis, being an independent prognosticator on multivariate analysis. Survivin nuclear positivity also correlated with younger age and lower incidence of vessel cancer invasion. In contrast, survivin cytoplasmic positivity was noted in 117 cases (88.0%) and did not correlate with any factor of progression or prognosis. The results indicate that survivin is present in the majority of gastric cancer cells but a nuclear localization may play an important physiological role in hindering tumor progression. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.