Active/de-active transition of respiratory complex I in bacteria, fungi, and animals
Active/de-active transition of respiratory complex I in bacteria, fungi, and animals
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DOI：
10.1016/s0005-2728(03)00087-2
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发表时间：
2003-09-30
影响因子：
4.3
通讯作者：
Cecchini, G
中科院分区：
文献类型：
--
作者：
Maklashina, E;Kotlyar, AB;Cecchini, G
哺乳动物复合物I（NADH：泛醌氧化还原酶）以可相互转化的活性（A）和失活（D）形式的混合物存在。A型能够进行NADH：醌还原酶催化，但D型不能。相比之下，副球菌的复合物I仅以A型存在。这种细菌复合体比动物复合体少32个亚基。因此，出现了这样的问题：高等生物的复合物I的结构复杂性是否与其经历A/D转变的能力相关。在本研究中，人们发现，复合物I从细菌大肠杆菌和非脊椎动物的生物体（蚯蚓，龙虾，蟋蟀）没有显示A/D转换。然而，脊椎动物（鲤鱼、青蛙、鸡）的A/D转换与牛复合物I的A/D转换相似。进一步的研究表明，复合物I从较低的真核生物，粗糙脉孢菌和解脂耶氏酵母，表现出非常明显的A/D转换与低得多的激活障碍相比，牛的酶。A/D转换的复杂的I，因为它们涉及到的结构和酶活性的调节进行了讨论。由爱思唯尔公司出版
Mammalian complex I (NADH:ubiquinone oxidoreductase) exists as a mixture of interconvertible active (A) and de-activated (D) forms. The A-form is capable of NADH:quinone-reductase catalysis, but not the D-form. Complex I from the bacterium Paracoccus denitrificans, by contrast, exists only in the A-form. This bacterial complex contains 32 fewer subunits than the manimalian complex. The question arises therefore if the structural complexity of complex I from higher organisms correlates with its ability to undergo the A/D transition. In the present study, it was found that complex I from the bacterium Escherichia coli and from non-vertebrate organisms (earthworm, lobster, and cricket) did not show the A/D transitions. Vertebrate organisms (carp, frog, chicken), however, underwent similar A/D transitions to those of the well-characterized bovine complex I. Further studies showed that complex I from the lower eukaryotes, Neurospora crassa and Yarrowia lipolytica, exhibited very distinct A/D transitions with much lower activation barriers compared to the bovine enzyme. The A/D transitions of complex I as they relate to structure and regulation of enzymatic activity are discussed. Published by Elsevier B.V.