A tissue-specific exon of myosin Va is responsible for selective cargo binding in melanocytes
A tissue-specific exon of myosin Va is responsible for selective cargo binding in melanocytes
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DOI：
10.1002/cm.10061
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发表时间：
2002-10-01
影响因子：
--
通讯作者：
Huang, JD
中科院分区：
文献类型：
--
作者：
Au, JSY;Huang, JD
V类肌球蛋白是用于细胞内运输和细胞器束缚的分子马达。小鼠肌球蛋白Va（MyoVa）由稀释基因座编码，其选择性剪接以产生几种组织特异性同种型。MyoVa的尾部是推定的货物结合结构域。为了确定MyoVa的不同同种型和最小货物结合区的功能，我们用绿色荧光蛋白标记小鼠MyoVa尾的各种同种型和不同部分，并检查它们在小鼠melan-a细胞中的细胞内定位。我们发现，编码的氨基酸序列的选择性剪接外显子，外显子F，是必要的选择性结合MyoVa黑素体。缺乏该氨基酸序列的MyoVa同种型不靶向黑素体，而是定位于核周区域。这些发现表明MyoVa能够结合多种类型的货物，选择性由可变剪接序列决定。细胞动力。Cytoskeleton 53：89-102，2002. (C)2002 Wiley-Liss，Inc.
Class V myosins are molecular motors used for intracellular transportation and organelle tethering. The mouse Myosin Va (MyoVa) is encoded by the dilute locus, which is alternatively spliced to generate several tissue specific isoforms. The tail of MyoVa is the putative cargo-binding domain. To determine the functions of different isoforms of MyoVa and the minimal cargo-binding region, we tagged various isoforms and different portions of the mouse MyoVa tail with a green fluorescent protein and examined their intracellular localizations in the mouse melan-a cells. We found that the amino acid sequence encoded by an alternatively spliced exon, exon F, is necessary for the selective binding of MyoVa to melanosome. The MyoVa isoforms lacking this amino acid sequence are not targeted to the melanosomes, but localized to the perinuclear region instead. These findings suggested that MyoVa is able to bind to more than one types of cargos, with the selectivities determined by alternative spliced sequences. Cell Motil. Cytoskeleton 53:89-102, 2002. (C) 2002 Wiley-Liss, Inc.