Jasmonic acid and salicylic acid induce accumulation of β-1,3-glucanase and thaumatin-like proteins in wheat and enhance resistance against Stagonospora nodorum
Jasmonic acid and salicylic acid induce accumulation of β-1,3-glucanase and thaumatin-like proteins in wheat and enhance resistance against Stagonospora nodorum
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DOI：
10.1023/b:biop.0000041097.03177.2d
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发表时间：
2004-01-01
影响因子：
1.5
通讯作者：
Velazhahan, R
中科院分区：
文献类型：
--
作者：
Jayaraj, J;Muthukrishnan, S;Velazhahan, R
研究了茉莉酸(JA)和水杨酸(SA)对小麦对条锈病抗性的诱导及对β-1，3-葡聚糖酶和类胡萝卜素蛋白(TLPs)的诱导作用。Western印迹分析表明，JA和SA处理小麦叶片后，诱导出两种表观分子量分别为31 kDa和33 kDa的β-1，3-葡聚糖酶，与大麦葡聚糖酶抗血清发生交叉反应。当SA和JA处理小麦植株时，诱导出一个表观分子质量为25 kDa的TLP和其他几种TLP的异构体。水杨酸和茉莉酸处理小麦植株后，与未处理对照相比，小麦植株的叶斑病发病率显著降低(最高可达56%)。
The effect of application of jasmonic acid (JA) and salicylic acid ( SA) on the induction of resistance in wheat to Stagonospora nodorum and on the induction of beta-1,3-glucanase and thaumatin-like proteins (TLPs) was studied. Western blot analysis revealed that two beta-1,3-glucanases with apparent molecular masses of 31 and 33 kDa that cross-reacted with a barley glucanase antiserum were induced in wheat leaves after treatment with JA and SA. When wheat plants were treated with SA and JA, a TLP with an apparent molecular mass of 25 kDa and several other isoforms of TLP were induced. Pre-treatment of wheat plants with SA and JA significantly reduced ( up to 56 %) the incidence of leaf blotch disease incited by S. nodorum compared with untreated control plants.