Direct proof of nitric oxide formation from a nitrovasodilator metabolised by erythrocytes.
Direct proof of nitric oxide formation from a nitrovasodilator metabolised by erythrocytes.
复制标题
红细胞代谢的硝基血管扩张剂形成一氧化氮的直接证据。
DOI：
10.1006/bbrc.1994.2569
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
T. Shiga
中科院分区：
文献类型：
--
作者：
H. Kosaka;S. Tanaka;T. Yoshii;E. Kumura;A. Seiyama;T. Shiga
向大鼠静脉注射15N-硝酸异山梨酯后，静脉血中NO(HbNO)的电子自旋共振(ESR)谱呈二线超精细结构，证明了15N-NO的产生。当人血与15N-硝酸异山梨酯厌氧混合时，ESR谱最初显示14N-HbNO，然后被增加的15N-HbNO淹没。在不加硝酸异山梨酯的情况下，对另外4名男性献血者的血液进行脱氧，其中两人有14N-HbNO，但其他人没有，这表明人血中存在不同数量的NO前体亚硝硫醇。N-乙基马来酰亚胺和谷胱甘肽-S转移酶抑制剂S-己基谷胱甘肽均不能抑制厌氧混合的溶血液中亚硝酸异山梨酯的生成。
When 15N-isosorbide dinitrate was injected into rats, the venous blood showed a 2-line hyperfine structure in the electron spin resonance (ESR) spectra of NO hemoglobin (HbNO), proving generation of 15N-NO. When human blood was mixed with 15N-isosorbide dinitrate anaerobically, ESR spectra showed initially 14N-HbNO, which was then overwhelmed with the increased 15N-HbNO. With deoxygenation of the blood of another 4 male donors without addition of isosorbide dinitrate, two showed 14N-HbNO, but others did not, suggesting varied amount of NO-precursers, nitrosothiols, in human blood. Neither N-ethylmaleimide nor S-hexylglutathione, a glutathione-S-transferase inhibitor, inhibited the formation of UbNO in the hemolysate mixed anaerobically with isosorbide dinitrite.