Unscheduled synthesis of DNA and poly(ADP-ribose) in human fibroblasts following DNA damage.
Unscheduled synthesis of DNA and poly(ADP-ribose) in human fibroblasts following DNA damage.
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DNA 损伤后，人成纤维细胞中 DNA 和聚（ADP-核糖）的非计划合成。
DOI：
10.1002/jsscb.380170110
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Jacobson,MK
中科院分区：
文献类型：
--
作者：
McCurry,LS;Jacobson,MK
在NAD转化为聚（ADP-核糖）的速率降低的条件下，在人成纤维细胞中测量了程序外DNA合成。着色性干皮病基因型杂合子细胞在聚（ADP-核糖）合成速率为正常细胞速率的一半的条件下，显示出UV诱导的程序外DNA合成的正常速率。在正常细胞的培养基中加入茶碱（一种有效的聚（ADP-核糖）聚合酶抑制剂），在用UV或N-甲基-N′-硝基-N-硝基-异胍处理后，阻断了超过90%的NAD转化为聚（ADP-核糖），但不影响程序外DNA合成的速率。
Unscheduled DNA synthesis has been measured in human fibroblasts under conditons of reduced rates of conversion of NAD to poly(ADP‐ribose). Cells heterozygous for the xeroderma pigmentosum genotype showed normal rates of UV induced unscheduled DNA synthesis under conditions in which the rate of poly(ADP‐ribose) synthesis was one‐half the rate of normal cells. The addition of theophylline, a potent inhibitor of poly(ADP‐ribose) polymerase, to the culture medium of normal cells blocked over 90% of the conversion of NAD to poly(ADP‐ribose) following treatment with UV or N‐methyl‐N′‐nitro‐N‐nitro‐soguanidine but did not affect the rate of unscheduled DNA synthesis.