Bisphenol A Exposure during Oocyte Maturation in vitro Results in Spindle Abnormalities and Chromosome Misalignment in Bos taurus
Bisphenol A Exposure during Oocyte Maturation in vitro Results in Spindle Abnormalities and Chromosome Misalignment in Bos taurus
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DOI：
10.1159/000381321
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发表时间：
2015-01-01
影响因子：
1.7
通讯作者：
King, W. Allan
中科院分区：
文献类型：
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作者：
Ferris, Jacqueline;Favetta, Laura A.;King, W. Allan
人类普遍接触双酚 A (BPA)，并且在包括卵泡液在内的多种样本中均检测到了 BPA。研究发现，BPA 水平与接受体外受精的女性卵母细胞的发育潜力呈负相关，并在人类和小鼠模型中通过实验诱导减数分裂异常。 BPA 可能会对卵母细胞的成熟产生不利影响，不同浓度的接触会导致不同的结果。由于卵母细胞成熟对发育潜力的重要性，在此期间的干扰会显着影响卵母细胞的活力。在这里，牛卵母细胞在培养基中经过或不经过 BPA 处理后在体外成熟。卵母细胞吸收的 BPA 水平远低于最初接触的水平。用 30 ng/ml 进行培养基处理后，成熟卵母细胞中测得的 BPA 平均含量为 2.48 ng/ml。这些卵母细胞表现出成熟度下降和纺锤体异常发生率增加。暴露于 30 ng/ml BPA 的卵母细胞中只有 57.4% 达到成熟，而对照组为 72.4%（p < 0.05）。与所有其他分析组相比，接触 BPA 后的成熟卵母细胞表现出异常纺锤体形态 (67.9%) 和染色体分散 (60%) 增加 (p < 0.05)。因此，在体外卵母细胞成熟过程中接触 BPA 有可能降低卵母细胞质量。 (C) 2015 S. Karger AG，巴塞尔
Bisphenol A (BPA) exposure in humans is widespread, and BPA has been detected in a variety of samples including follicular fluid. BPA levels have been found to negatively correlate with the developmental potential of oocytes in women undergoing in vitro fertilization and to induce meiotic abnormalities experimentally in human and mouse models. BPA may detrimentally affect oocyte maturation, and different concentrations of exposure can cause various outcomes. Because of the importance of oocyte maturation on developmental potential, disturbances during this time can significantly impact oocyte viability. Here, bovine oocytes were matured in vitro with and without BPA treatment of the media. The levels of BPA taken up by the oocytes were much lower than the initial exposure. Medium treatment with 30 ng/ml resulted in an average of 2.48 ng/ml BPA measured in mature oocytes. These oocytes exhibited decreased maturation and increased incidence of spindle abnormalities. Only 57.4% of oocytes exposed to 30 ng/ml BPA reached maturity compared to 72.4% of controls (p < 0.05). Mature oocytes following BPA exposure displayed increased abnormal spindle morphology (67.9%) and chromosome dispersal (60%) compared to all other groups analyzed (p < 0.05). Thus, exposure to BPA during in vitro oocyte maturation has the potential to decrease oocyte quality. (C) 2015 S. Karger AG, Basel