Common regions of deletion on chromosome 22q12.3-q13.1 and 22q13.2 in human astrocytomas appear related to malignancy grade
Common regions of deletion on chromosome 22q12.3-q13.1 and 22q13.2 in human astrocytomas appear related to malignancy grade
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DOI：
10.1097/00005072-199908000-00010
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发表时间：
1999-08-01
影响因子：
3.2
通讯作者：
Louis, DN
中科院分区：
文献类型：
--
作者：
Ino, Y;Silver, JS;Louis, DN
据报道，大约30%的人类星形细胞瘤显示出22号染色体长臂的等位基因缺失，这表明存在22q号染色体星形细胞瘤抑制基因。为了确定这个假定的肿瘤抑制基因最可能的位置，我们使用16个染色体22q微卫星标记对141个肿瘤进行了缺失定位。在2/12(17%)的星形细胞瘤、9/29(31%)的间变性星形细胞瘤和38/100(38%)的胶质母细胞瘤中观察到22q的等位基因缺失，这与染色体22q缺失在星形细胞瘤的发展和形成中的作用是一致的。22个肿瘤在每个信息位点都表现出等位基因丢失，这与22q的整个手臂的丢失一致。27例肿瘤有部分缺失，其中一个共同缺失区域位于标记D22S280和D22S282之间的22q12.3-q13.1，第二个候选区域位于标记D22S1170附近的22q13.2。对于近端候选区域，等位基因丢失的发生率在不同年级之间相似；对于远端基因座，随着级别的增加，等位基因缺失的发生率增加，增加了该基因座参与星形细胞瘤发生的后期阶段的可能性。
Approximately 30% of human astrocytomas have been reported to display allelic loss of the long arm of chromosome 22, suggesting the presence of a chromosome 22q astrocytoma suppressor gene. To define the most likely location for this putative tumor suppressor, we performed deletion mapping on 141 tumors using 16 chromosome 22q microsatellite markers. Allelic loss of 22q was observed in 2/12 (17%) of astrocytomas, 9/29 (31%) of anaplastic astrocytomas, and 38/100 (38%) of glioblastomas, consistent with a role for chromosome 22q loss in astrocytoma progression as well as formation. Twenty-two tumors exhibited allelic loss at every informative locus, consistent with loss of the entire arm of 22q. Twenty-seven tumors showed partial deletions, with one common region of deletion at 22q12.3-q13.1 between markers D22S280 and D22S282, and a second candidate region at 22q13.2 near the marker D22S1170. For the proximal candidate region, the incidence of allelic loss was similar between grades; for the distal locus, the incidence increased with grade, raising the possibility that the distal locus is involved in a later stage of astrocytoma tumorigenesis.