Caspase-1 activity is required to bypass macrophage apoptosis upon Salmonella infection.
Caspase-1 activity is required to bypass macrophage apoptosis upon Salmonella infection.
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DOI：
10.1038/nchembio.1023
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发表时间：
2012-09
影响因子：
14.8
通讯作者：
中科院分区：
文献类型：
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在这里，我们报告AWP 28，一种基于活性的探针，可用于生化监测响应促炎刺激的caspase-1激活。使用AWP 28，我们表明，在缺乏半胱天冬酶-1的原代小鼠骨髓巨噬细胞中，细菌感染后会触发细胞凋亡。此外，我们报告说，沙门氏菌感染后，炎性小体介导的半胱天冬酶-1活性需要绕过细胞凋亡，有利于促炎性细胞死亡。
Here we report AWP28, an activity-based probe that can be used to biochemically monitor caspase-1 activation in response to pro-inflammatory stimuli. Using AWP28 we show that apoptosis is triggered upon bacterial infection in primary murine bone marrow macrophages lacking caspase-1. Furthermore we report that upon Salmonella infection, inflammasome-mediated caspase-1 activity is required to bypass apoptosis in favor of pro-inflammatory pyroptotic cell death.