Structure of the MecI repressor from Staphylococcus aureus in complex with the cognate DNA operator of mec.
Structure of the MecI repressor from Staphylococcus aureus in complex with the cognate DNA operator of mec.
复制标题
金黄色葡萄球菌的 MecI 阻遏蛋白与 mec 的同源 DNA 操纵子复合的结构。
DOI：
10.1107/s1744309106009742
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Archer,GordonL
中科院分区：
文献类型：
--
作者：
Safo,MartinK;Ko,TzuPing;Musayev,FaikN;Zhao,Qixun;Wang,AndrewHJ;Archer,GordonL
The dimeric repressor MecI regulates the mecA gene that encodes the penicillin-binding protein PBP-2a in methicillin-resistant Staphylococcus aureus (MRSA). MecI is similar to BlaI, the repressor for the blaZ gene of β-lactamase. MecI and BlaI can bind to both operator DNA sequences. The crystal structure of MecI in complex with the 32 base-pair cognate DNA of mec was determined to 3.8 Å resolution. MecI is a homodimer and each monomer consists of a compact N-terminal winged-helix domain, which binds to DNA, and a loosely packed C-terminal helical domain, which intertwines with its counter-monomer. The crystal contains horizontal layers of virtual DNA double helices extending in three directions, which are separated by perpendicular DNA segments. Each DNA segment is bound to two MecI dimers. Similar to the BlaI–mec complex, but unlike the MecI–bla complex, the MecI repressors bind to both sides of the mec DNA dyad that contains four conserved sequences of TACA/TGTA. The results confirm the up-and-down binding to the mec operator, which may account for cooperative effect of the repressor.
BlaI 的溶液结构研究：对抑制与 β-内酰胺抗生素耐药性有关的基因的影响。
影响因子：
5.6
作者：
H. V. Melckebeke;C. Vreuls;P. Gans;P. Filée;G. Llabrès;B. Joris;J. Simorre
通讯作者：
J. Simorre
MecI的三维结构
影响因子：
4.8
作者：
R. García;A. Marrero;G. Mallorquí;J. Potempa;M. Coll;F. Gomis
通讯作者：
F. Gomis
影响因子：
4.8
作者：
R. García;G. Mallorquí;A. Marrero;J. Potempa;M. Coll;F. Gomis
通讯作者：
F. Gomis