Influence of host phylogeny and water physicochemistry on microbial assemblages of the fish skin microbiome
Influence of host phylogeny and water physicochemistry on microbial assemblages of the fish skin microbiome
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DOI：
10.1093/femsec/fiae021
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发表时间：
2024-02-14
影响因子：
4.2
通讯作者：
Tyler，Charles R.
中科院分区：
文献类型：
--
作者：
Bell，Ashley G.;McMurtrie，Jamie;Tyler，Charles R.
鱼的皮肤含有多种微生物群，与宿主具有共生功能，促进病原体排除，免疫系统启动和营养物质降解。鱼皮微生物组的组成在不同物种和对各种压力源的反应中有所不同，然而，这些研究中没有系统的分析来评估这些因素如何塑造鱼皮微生物组。在这里，我们研究了来自36项研究的1922个鱼皮微生物组，包括98个鱼种和9种饲养条件，以研究鱼皮微生物组、鱼类和水理化因素之间的关系。变形菌纲，特别是伽马变形菌纲，存在于所有海洋和淡水鱼皮肤微生物群中。淡水鱼皮肤微生物群中以不动杆菌属、气单胞菌属、拉尔斯顿菌属、鱼单胞菌属和黄杆菌属数量最多；咸水鱼皮肤微生物群中以互变单胞菌属、光杆菌属、假互变单胞菌属、冷杆菌属和弧菌属数量最多。结果表明，不同的培养（饲养）环境对皮肤细菌群落组成的影响虽小但显著。水温、pH、溶解氧浓度和盐度与β -多样性差异显著相关，但与α -多样性差异无关。为了提高鱼类皮肤微生物组研究的可比性，我们提出了测序数据分析方法和提高研究可重复性的建议。
The skin of fish contains a diverse microbiota that has symbiotic functions with the host, facilitating pathogen exclusion, immune system priming, and nutrient degradation. The composition of fish skin microbiomes varies across species and in response to a variety of stressors, however, there has been no systematic analysis across these studies to evaluate how these factors shape fish skin microbiomes. Here, we examined 1922 fish skin microbiomes from 36 studies that included 98 species and nine rearing conditions to investigate associations between fish skin microbiome, fish species, and water physiochemical factors. Proteobacteria, particularly the class Gammaproteobacteria, were present in all marine and freshwater fish skin microbiomes.Acinetobacter, Aeromonas, Ralstonia, SphingomonasandFlavobacteriumwere the most abundant genera within freshwater fish skin microbiomes, andAlteromonas, Photobacterium, Pseudoalteromonas, PsychrobacterandVibriowere the most abundant in saltwater fish. Our results show that different culturing (rearing) environments have a small but significant effect on the skin bacterial community compositions. Water temperature, pH, dissolved oxygen concentration, and salinity significantly correlated with differences in beta-diversity but not necessarily alpha-diversity. To improve study comparability on fish skin microbiomes, we provide recommendations for approaches to the analyses of sequencing data and improve study reproducibility.