Nuclear magnetic resonance spectroscopy of the circadian clock of cyanobacteria.
Nuclear magnetic resonance spectroscopy of the circadian clock of cyanobacteria.
复制标题
蓝藻生物钟的核磁共振波谱。
DOI：
10.1093/icb/ict054
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发表时间：
2013
影响因子：
2.6
通讯作者：
LiWang,Andy
中科院分区：
文献类型：
--
作者：
Chang,Yong-Gang;Tseng,Roger;Kuo,Nai-Wei;LiWang,Andy
在分子机制水平上，最为人熟知的生物钟是蓝藻。本文概述了溶液状态核磁共振（NMR）光谱如何有助于这种理解。通过在强磁场中激发自旋为1/2的原子核，NMR获得了有关其化学环境、核间距离、方向和运动的信息。NMR蛋白质样品通常是水性的，通常在接近生理pH、离子强度和温度下。通过NMR可获得的信息水平取决于NMR样品的质量，我们指的是蛋白质的溶解度和稳定性。在这里，我们使用我们实验室的例子来说明该技术的优点和局限性。
The most well-understood circadian clock at the level of molecular mechanisms is that of cyanobacteria. This overview is on how solution-state nuclear magnetic resonance (NMR) spectroscopy has contributed to this understanding. By exciting atomic spin-½ nuclei in a strong magnetic field, NMR obtains information on their chemical environments, inter-nuclear distances, orientations, and motions. NMR protein samples are typically aqueous, often at near-physiological pH, ionic strength, and temperature. The level of information obtainable by NMR depends on the quality of the NMR sample, by which we mean the solubility and stability of proteins. Here, we use examples from our laboratory to illustrate the advantages and limitations of the technique.