Human rhabdomyosarcoma cell lines for rhabdomyosarcoma research: utility and pitfalls.
Human rhabdomyosarcoma cell lines for rhabdomyosarcoma research: utility and pitfalls.
复制标题
横纹肌肉瘤研究的人类横纹肌肉瘤细胞系研究：效用和陷阱。
DOI：
10.3389/fonc.2013.00183
复制
发表时间：
2013
影响因子：
4.7
通讯作者：
Linardic CM
中科院分区：
文献类型：
--
作者：
Hinson AR;Jones R;Crose LE;Belyea BC;Barr FG;Linardic CM
横纹肌肉瘤是儿童和青少年最常见的软组织肉瘤。尽管在欧洲和北美进行了组间临床试验，但在过去几十年中，患有这种疾病的高风险患者的结局没有显著改善，转移性或复发性疾病的生存率仍然非常低。进入新的临床试验是缓慢而困难的，因此体外细胞系研究和体内异种移植模型为这种癌症类型的临床前研究提供了一种有吸引力的替代方案。目前，存在30种常用的人RMS细胞系，具有不同的起源、核型、组织学和验证方法。选择合适的细胞系进行RMS研究对结果具有重要意义。在使用某些细胞系时也存在潜在的缺陷，包括鼠基质细胞的污染、细胞系之间的交叉污染、细胞系与其相关的原始肿瘤之间的不一致、冒名顶替的细胞系以及导致实际上来自相同来源的两种或更多种假定的独特细胞系的循环的命名错误。这些缺陷可以通过检测物种特异性同工酶、微阵列分析、亚型特异性融合产物测定和短串联重复序列分析来避免。
Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma of childhood and adolescence. Despite intergroup clinical trials conducted in Europe and North America, outcomes for high risk patients with this disease have not significantly improved in the last several decades, and survival of metastatic or relapsed disease remains extremely poor. Accrual into new clinical trials is slow and difficult, so in vitro cell-line research and in vivo xenograft models present an attractive alternative for preclinical research for this cancer type. Currently, 30 commonly used human RMS cell lines exist, with differing origins, karyotypes, histologies, and methods of validation. Selecting an appropriate cell line for RMS research has important implications for outcomes. There are also potential pitfalls in using certain cell lines including contamination with murine stromal cells, cross-contamination between cell lines, discordance between the cell line and its associated original tumor, imposter cell lines, and nomenclature errors that result in the circulation of two or more presumed unique cell lines that are actually from the same origin. These pitfalls can be avoided by testing for species-specific isoenzymes, microarray analysis, assays for subtype-specific fusion products, and short tandem repeat analysis.