Negative visualization of DNA in agarose gel by eosin Y and its related mechanism
Negative visualization of DNA in agarose gel by eosin Y and its related mechanism
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曙红Y对琼脂糖凝胶中DNA的负显像及其相关机制
DOI：
10.1039/c2an00008c
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发表时间：
2012-01-01
期刊：
影响因子：
4.2
通讯作者：
Li, Xiaokun
中科院分区：
文献类型：
--
作者：
Cong, Weitao;You, Weijing;Li, Xiaokun
我们在这里报告一个敏感和环境友好的技术，通过使用曙红Y，这似乎是一个透明的和无色的带下不透明的凝胶基质背景下的DNA可视化。低至0.2-0.6 ng的DNA可以在1小时内可视化，类似于最常用的溴化乙锭染色。并对新方法的机理进行了阐述。结果表明，曙红Y与DNA的复合物主要是通过沟结合作用形成的，而不是嵌入DNA的双螺旋结构中。
We report here a sensitive and environmentally benign technique for the visualization of DNA by using eosin Y, which appeared as a transparent and colorless band under the opaque gel matrix background. As low as 0.2-0.6 ng of DNA can be visualized within 1 h, similar to that of the most commonly used ethidium bromide stain. Furthermore, the mechanism of the newly developed method was also described. According to the results, it can be concluded that the complex between DNA and eosin Y is mainly formed by the function of groove binding, but not intercalate itself into the double helix structure of DNA.