Post-occlusive reactive hyperemia and skeletal muscle capillary hemodynamics.
Post-occlusive reactive hyperemia and skeletal muscle capillary hemodynamics.
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卵巢后反应性充血和骨骼肌毛细血管血液动力学。
DOI：
10.1016/j.mvr.2021.104283
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发表时间：
2022-03
影响因子：
3.1
通讯作者：
Behnke BJ
中科院分区：
文献类型：
--
作者：
Horn AG;Schulze KM;Weber RE;Barstow TJ;Musch TI;Poole DC;Behnke BJ
闭塞后反应性充血（PORH）是评估外周大血管功能的公认诊断工具。虽然管道动脉血流动力学已被很好地定义，但PORH对毛细血管血流动力学的影响仍然未知，尽管微血管是血管控制的主要部位。因此，本研究的目的是确定供血动脉阻断5分钟对骨骼肌毛细血管血流动力学的影响。我们测试了以下假设：在动脉闭塞解除后，与闭塞前条件相比，将存在：1）增加的红细胞通量（fRBC）和红细胞速度（VRBC），以及2）减少的支持RBC流动的毛细血管比例。在雌性Sprague-Dawley大鼠（n = 6）中，将脊髓背侧肌外置以评价闭塞前、供血动脉闭塞5分钟（Occ）和再灌注5分钟（Post-Occ）的毛细血管血流动力学。平均动脉压（MAP）和毛细血管直径（Dc）在阻塞前和阻塞后均无显著性差异（P > 0.05）。在PORH 30 s时，毛细血管fRBC增加（术前：59 ± 4 vs 30 s后：77 ± 2 cells/s; P < 0.05），VRBC无变化（术前：300 ± 24 vs 30 s后：322 ± 25 μm/s; P > 0.05）。毛细血管红细胞压积（Hctcap）在阻塞前至阻塞后条件下无变化（P > 0.05）。闭塞后30 s和300 s-闭塞后，支持RBC流动的毛细血管数量减少20-30%（前：92 ± 2%; 30 s-闭塞后：66 ± 3%; 300 s-闭塞后：72 ± 6%;均P < 0.05）。短期供血动脉闭塞（即5分钟）导致更不均匀的毛细血管流量曲线与毛细血管无复流的存在下，减少支持RBC流量的毛细血管的百分比。小动脉水平的生肌和代谢机制之间的复杂相互作用可能在PORH的毛细血管无复流中发挥作用。在导管动脉水平的测量掩盖了微循环中血流分布的显著变化。
Post-occlusive reactive hyperemia (PORH) is an accepted diagnostic tool for assessing peripheral macrovascular function. While conduit artery hemodynamics have been well defined, the impact of PORH on capillary hemodynamics remains unknown, despite the microvasculature being the dominant site of vascular control. Therefore, the purpose of this investigation was to determine the effects of 5 minutes of feed artery occlusion on capillary hemodynamics in skeletal muscle. We tested the hypothesis that, upon release of arterial occlusion, there would be: 1) an increased red blood cell flux (fRBC) and red blood cell velocity (VRBC), and 2) a decreased proportion of capillaries supporting RBC flow compared to the pre-occlusion condition. In female Sprague-Dawley rats (n = 6), the spinotrapezius muscle was exteriorized for evaluation of capillary hemodynamics pre-occlusion, 5 min of feed artery occlusion (Occ), and 5 min of reperfusion (Post-Occ). There were no differences in mean arterial pressure (MAP) or capillary diameter (Dc) between pre-occlusion and post-occlusion (P > 0.05). During 30s of PORH, capillary fRBC was increased (pre: 59 ± 4 vs 30s-post: 77 ± 2 cells/s; P < 0.05) and VRBC was not changed (pre: 300 ± 24 vs 30s post: 322 ± 25 μm/s; P > 0.05). Capillary hematocrit (Hctcap) was unchanged across the pre- to post-occlusion conditions (P > 0.05). Following occlusion, there was a 20–30% decrease in the number of capillaries supporting RBC flow at 30s and 300s-post occlusion (pre: 92 ± 2%; 30s-post: 66 ± 3%; 300s-post: 72 ± 6%; both P < 0.05). Short-term feed artery occlusion (i.e. 5 min) resulted in a more heterogeneous capillary flow profile with the presence of capillary no-reflow, decreasing the percentage of capillaries supporting RBC flow. A complex interaction between myogenic and metabolic mechanisms at the arteriolar level may play a role in the capillary no-reflow with PORH. Measurements at the level of the conduit artery mask significant alterations in blood flow distribution in the microcirculation.
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影响因子：
20.1
作者：
FABER, JE
通讯作者：
FABER, JE
DOI：
10.1016/s0967-2109(02)00070-4
发表时间：
2002-12-01
期刊：
CARDIOVASCULAR SURGERY
影响因子：
--
作者：
Blaisdell, FW
通讯作者：
Blaisdell, FW
影响因子：
37.8
作者：
Anderson, Todd J.;Charbonneau, Francois;Lonn, Eva M.
通讯作者：
Lonn, Eva M.
影响因子：
37.8
作者：
Groebe, K
通讯作者：
Groebe, K
影响因子：
2.4
作者：
Ghonaim，Nour W.;Fraser，Graham M.;Ellis，Christopher G.
通讯作者：
Ellis，Christopher G.