Identification of RAD16, a yeast excision repair gene homologous to the recombinational repair gene RAD54 and to the SNF2 gene involved in transcriptional activation.
Identification of RAD16, a yeast excision repair gene homologous to the recombinational repair gene RAD54 and to the SNF2 gene involved in transcriptional activation.
复制标题
鉴定 RAD16，这是一种酵母切除修复基因，与重组修复基因 RAD54 和参与转录激活的 SNF2 基因同源。
DOI：
10.1002/yea.320080506
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Laurent,BC
中科院分区：
文献类型：
--
作者：
Schild,D;Glassner,BJ;Mortimer,RK;Carlson,M;Laurent,BC
TheRAD54gene ofSaccharomyces cerevisiaeis involved in the recombinational repair of DNA damage. The predicted amino acid sequence of the RAD54 protein shows significant homologies with the yeast SNF2 protein, which is required for the transcriptioal activation of a number of diversely regulated genes. These proteins are 31% identical in a 492‐amino acid region that includes presumed nucleotide and Mg2+binding sites. We noted previously that the SNF2 protein also shares homology with a partial open reading frame (ORF) that was reported with the sequence of an adjacent gene. This ORF also shares homology with the RAD54 protein. To test whether this ORF is involved in transcriptional activation or DNA repair, yeast strains deleted for part of it have been isolated. These strains do not show a Snf‐like phenotyp, but they are UV sensitive. This gene has been identified asRAD 16, a gene involved in the excision repair of DNA damage. Analysis of therad16deletion mutations indicates thatRAD16encodes a nonessential function and is not absolutely required for excision repair. Outside the region of homology to RAD54 and SNF2, the predicted RAD16 protein contains a novel cysteine‐rich motif that may bind zinc and that has been found recently in eleven other proteins, including the yeast RAD18 protein. The homologies betweenRAD16, RAD54andSNF2are also shared by several additional, recently isolated yeast andDrosophila genes.
